Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Falnes, P.O.; Johansen, R.F.; Seeberg, E.
    AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli (2002), Nature, 419, 178-182.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.33 expressed in Escherichia coli BL21 Star(DE3)pLysS cells Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.33 Fe2+ required for activity Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.33 Escherichia coli
-
-
-
1.14.11.33 Escherichia coli W3110 / ATCC 27325
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.33 GSTrap column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.33 DNA-1-methyladenine + 2-oxoglutarate + O2
-
 Escherichia coli DNA-adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-1-methyladenine + 2-oxoglutarate + O2
-
 Escherichia coli W3110 / ATCC 27325 DNA-adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-3-methylcytosine + 2-oxoglutarate + O2
-
 Escherichia coli DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-3-methylcytosine + 2-oxoglutarate + O2
-
 Escherichia coli W3110 / ATCC 27325 DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 methylated single-stranded poly(deoxyadenosine) + 2-oxoglutarate + O2
-
 Escherichia coli single-stranded poly(deoxyadenosine) + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 methylated single-stranded poly(deoxyadenosine) + 2-oxoglutarate + O2
-
 Escherichia coli W3110 / ATCC 27325 single-stranded poly(deoxyadenosine) + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 additional information although the lesions targeted by AlkB are introduced primarily into single-stranded DNA, AlkB is also capable of removing lesions from double-stranded DNA Escherichia coli ?
-
 ?
1.14.11.33 additional information although the lesions targeted by AlkB are introduced primarily into single-stranded DNA, AlkB is also capable of removing lesions from double-stranded DNA Escherichia coli W3110 / ATCC 27325 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.33 AlkB
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.33 2-oxoglutarate
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
1.14.11.33 physiological function AlkB-mediated oxidative demethylation reverses DNA damage Escherichia coli