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Literature summary extracted from
- Oxidative demethylation by Escherichia coli AlkB directly reverts DNA base damage (2002), Nature, 419, 174-178.
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.33 | Fe2+ | required for activity | Escherichia coli |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.11.33 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.11.33 | - |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.33 | DNA-1-methyladenine + 2-oxoglutarate + O2 | purified AlkB repairs the cytotoxic lesion 1-methyladenine in single- and double-stranded DNA | Escherichia coli | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | DNA-3-methylcytosine + 2-oxoglutarate + O2 | purified AlkB repairs the cytotoxic lesion 3-methylcytosine in single- and double-stranded DNA | Escherichia coli | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | methylated poly(deoxyadenine) + 2-oxoglutarate + O2 | 0.1 pmol AlkB protein removes 1.7 pmol 1-methyladenine from methylated poly(deoxyadenine) in 15 min | Escherichia coli | poly(deoxyadenine) + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | methylated poly(deoxycytosine) + 2-oxoglutarate + O2 | - |
Escherichia coli | poly(deoxycytosine) + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | additional information | AlkB has no detectable nuclease, DNA glycosylase or methyltransferase activity | Escherichia coli | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.11.33 | AlkB | - |
Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.33 | 2-oxoglutarate | - |
Escherichia coli | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.11.33 | malfunction | Escherichia coli alkB mutants are defective in processing methylation damage generated in single-stranded DNA | Escherichia coli |
| 1.14.11.33 | physiological function | oxidative demethylation by Escherichia coli AlkB directly reverts DNA base damage | Escherichia coli |
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Release 2023.1 (January 2023)
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