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Literature summary extracted from

  • Chatterjee, A.; Li, Y.; Zhang, Y.; Grove, T.L.; Lee, M.; Krebs, C.; Booker, S.J.; Begley, T.P.; Ealick, S.E.
    Reconstitution of ThiC in thiamine pyrimidine biosynthesis expands the radical SAM superfamily (2008), Nat. Chem. Biol., 4, 758-765.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.17 expression in Escherichia coli Caulobacter vibrioides

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.99.17 selenomethionine HMP-P synthase/HMP crystals are grown using the sitting drop vapor diffusion method at 25°C. The crystals are monoclinic, space group P2(1), with unit cell dimension a = 63.3 A, b = 103.4 A, c = 95.4 A and beta = 91.6° Caulobacter vibrioides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.99.17 4Fe-4S center binds 1 4Fe-4S cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.17 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine Caulobacter vibrioides the enzyme is involved in the biosynthesis of thiamine diphosphate 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
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Organism

EC Number Organism UniProt Comment Textmining
4.1.99.17 Caulobacter vibrioides Q9A6Q5
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.17 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine
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Caulobacter vibrioides 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
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?
4.1.99.17 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine the enzyme is involved in the biosynthesis of thiamine diphosphate Caulobacter vibrioides 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
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?

Subunits

EC Number Subunits Comment Organism
4.1.99.17 homodimer
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Caulobacter vibrioides

Synonyms

EC Number Synonyms Comment Organism
4.1.99.17 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase
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Caulobacter vibrioides
4.1.99.17 HMP-P synthase
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Caulobacter vibrioides
4.1.99.17 thiC
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Caulobacter vibrioides

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.17 [4Fe-4S]-center binds 1 4Fe-4S cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine Caulobacter vibrioides

General Information

EC Number General Information Comment Organism
4.1.99.17 physiological function the enzyme is involved in the biosynthesis of thiamine diphosphate Caulobacter vibrioides