Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jiang, J.; He, X.; Cane, D.E.
    Biosynthesis of the earthy odorant geosmin by a bifunctional Streptomyces coelicolor enzyme (2007), Nat. Chem. Biol., 3, 711-715.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.22 0.000062
-
 (2E,6E)-farnesyl diphosphate pH 8.2, 30°C Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.22 Streptomyces coelicolor Q9X839 germacradienol/germacrene D synthase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.22 (2E,6E)-farnesyl diphosphate + H2O
-
 Streptomyces coelicolor (1E,4S,5E,7R)-germa-1(10),5-dien-11-ol + diphosphate catalysed by the N-terminal domain of the bifunctional enzyme ?

Subunits

EC Number Subunits Comment Organism
4.2.3.22 More the recombinant N-terminal half of the protein catalyzes the Mg2+-dependent cyclization of farnesyl diphosphate to germacradienol and germacrene D, while the highly homologous C-terminal domain catalyzes the Mg2+-dependent conversion of germacradienol to geosmin Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
4.2.3.22 Cyc2
-
 Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.22 0.0062
-
 (2E,6E)-farnesyl diphosphate pH 8.2, 30°C Streptomyces coelicolor

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.22 100
-
 (2E,6E)-farnesyl diphosphate pH 8.2, 30°C Streptomyces coelicolor