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Literature summary extracted from

  • Wright, D.; DeBeaux, A.; Shi, R.; Doherty, A.J.; Harrison, L.
    Characterization of the roles of the catalytic domains of Mycobacterium tuberculosis ligase D in Ku-dependent error-prone DNA end joining (2010), Mutagenesis, 25, 473-481.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 expressed in Escherichia coli BW23474 cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.1 H373A mutant without 3'-5' single-stranded DNA exonuclease activity Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Mg2+ LigD has 3'-5' single-stranded DNA exonuclease activity that requires magnesium or manganese Mycobacterium tuberculosis
6.5.1.1 Mn2+ LigD has 3'-5' single-stranded DNA exonuclease activity that requires magnesium or manganese Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Mycobacterium tuberculosis AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?
6.5.1.1 additional information the PolDom domain of LigD is required for efficient and accurate DNA repair and increases deletions in re-circularized plasmid DNA when expressed with Ku protein in Escherichia coli. The LigDom domain of LigD can function in Ku-dependent repair Mycobacterium tuberculosis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.1 ? x * about 100000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 adenosine triphosphate-dependent ligase
-
 Mycobacterium tuberculosis
6.5.1.1 ATP-dependent ligase
-
 Mycobacterium tuberculosis
6.5.1.1 ligase D
-
 Mycobacterium tuberculosis
6.5.1.1 LigD
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.1 ATP
-
 Mycobacterium tuberculosis

Expression

EC Number Organism Comment Expression
6.5.1.1 Mycobacterium tuberculosis LigD protein is only expressed when 0.2% L-arabinose is added to the culture up