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Literature summary extracted from

  • Rouse, D.A.; DeVito, J.A.; Li, Z.; Byer, H.; Morris, S.L.
    Site-directed mutagenesis of the katG gene of Mycobacterium tuberculosis: effects on catalase-peroxidase activities and isoniazid resistance (1996), Mol. Microbiol., 22, 583-592.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.21 expressed in Escherichia coli JM109 cells and in Mycobacterium bovis BCG Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.21 A350T the catalase and peroxidase activities and isoniazid sensitivity is similar to the wild type enzyme Mycobacterium tuberculosis
1.11.1.21 D381G the mutant shows no catalase activity Mycobacterium tuberculosis
1.11.1.21 H108Q the mutant shows no catalase activity Mycobacterium tuberculosis
1.11.1.21 H270Q the mutant shows no catalase activity Mycobacterium tuberculosis
1.11.1.21 L148R the mutant shows no catalase activity Mycobacterium tuberculosis
1.11.1.21 L587M the catalase and peroxidase activities and isoniazid sensitivity is similar to the wild type enzyme Mycobacterium tuberculosis
1.11.1.21 N138S the KatG level of the mutant is 30% of wild type level, the mutant shows a 90% reduction in peroxidase activity Mycobacterium tuberculosis
1.11.1.21 R104L mutant with reduced enzymatic activity Mycobacterium tuberculosis
1.11.1.21 R463G the catalase and peroxidase activities and isoniazid sensitivity is similar to the wild type enzyme Mycobacterium tuberculosis
1.11.1.21 R463L the catalase and peroxidase activities and isoniazid sensitivity is similar to the wild type enzyme Mycobacterium tuberculosis
1.11.1.21 S140N the catalase and peroxidase activities and isoniazid sensitivity is similar to the wild type enzyme Mycobacterium tuberculosis
1.11.1.21 S315T the KatG level of the mutant is 90% of wild type level, the mutant shows 60% peroxidase and 40% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
1.11.1.21 T275P the mutant shows no catalase activity Mycobacterium tuberculosis
1.11.1.21 W321G the KatG level of the mutant is 40% of wild type level, the mutant shows a 70% reduction in peroxidase and catalase activities Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.21 Mycobacterium tuberculosis
-
-
-
1.11.1.21 Mycobacterium tuberculosis H37Rv
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Mycobacterium tuberculosis oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Mycobacterium tuberculosis H37Rv oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.1.21 H2O2
-
 Mycobacterium tuberculosis O2 + H2O
-
 ?
1.11.1.21 H2O2
-
 Mycobacterium tuberculosis H37Rv O2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.21 KatG
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.21 heme
-
 Mycobacterium tuberculosis