EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.22 | expressed in Escherichia coli and in Methylotrophic yeast Ogataea minuta TK5-3/Ura cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.22 | M51I | enzymological parameter values are almost the same as those of the wild-type GLA. Km (4-methylumbelliferyl alpha-D-galactoside) similar to wild-type. Mutant enzyme is unstable under neutral pH conditions and acidic ones. The effect of imino sugars including 1-deoxygalactonojirimycin and galactostatin bisulfite on the purified mutant enzyme is examined. The imino sugars improve the stability of the mutant enzyme under both neutral and acidic pH conditions. The results of surface plasmon resonance biosensor assaying suggests that the imino sugars retain their binding activity as to the mutant enzyme under both neutral and acidic pH conditions | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | 1-deoxygalactonojirimycin | - |
Homo sapiens | |
3.2.1.22 | galactostatin bisulfite | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.22 | 4 | - |
4-methylumbelliferyl alpha-D-galactoside | pH 4.6, 37°C, mutant M51I | Homo sapiens | |
3.2.1.22 | 4.5 | - |
4-methylumbelliferyl alpha-D-galactoside | pH 4.6, 37°C, wild-type | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.22 | Homo sapiens | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.22 | 4-methylumbelliferyl alpha-D-galactoside + H2O | - |
Homo sapiens | 4-methylumbelliferone + D-galactose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.22 | alpha-galactosidase | - |
Homo sapiens |
3.2.1.22 | GLA | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 37 | - |
- |
Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 4.6 | - |
- |
Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.22 | 0.038 | - |
1-deoxygalactonojirimycin | pH 4.6, 37°C, wild-type | Homo sapiens | |
3.2.1.22 | 0.047 | - |
1-deoxygalactonojirimycin | pH 4.6, 37°C, mutant M51I | Homo sapiens | |
3.2.1.22 | 0.095 | - |
galactostatin bisulfite | pH 4.6, 37°C, wild-type | Homo sapiens | |
3.2.1.22 | 0.122 | - |
galactostatin bisulfite | pH 4.6, 37°C, mutant M51I | Homo sapiens |