EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.3 | expressed in Escherichia coli M15 cells | uncultured bacterium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | Ca2+ | 49% residual activity at 1 mM | uncultured bacterium | |
3.1.1.3 | Cu2+ | complete inhibition at 0.1 mM | uncultured bacterium | |
3.1.1.3 | diethyl dicarbonate | complete inhibition at 0.1 mM | uncultured bacterium | |
3.1.1.3 | Hg2+ | complete inhibition at 0.1 mM | uncultured bacterium | |
3.1.1.3 | Li+ | 88% residual activity at 1 mM | uncultured bacterium | |
3.1.1.3 | Mg2+ | 62% residual activity at 0.1 mM | uncultured bacterium | |
3.1.1.3 | Mn2+ | 92% residual activity at 1 mM | uncultured bacterium | |
3.1.1.3 | additional information | not inhibited by phenylmethylsulfonyl fluoride, eserine, and SDS | uncultured bacterium | |
3.1.1.3 | Triton X-100 | the protein retains about 70% activity with Triton X-100 | uncultured bacterium | |
3.1.1.3 | Zn2+ | 58% residual activity at 1 mM | uncultured bacterium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.3 | 0.00073 | - |
4-nitrophenyl laurate | pH 8.0, 50°C | uncultured bacterium |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | Ca2+ | 163% activity at 0.1 mM | uncultured bacterium | |
3.1.1.3 | Co2+ | 124% activity at 0.1 mM | uncultured bacterium | |
3.1.1.3 | Li+ | 112% activity at 0.1 mM | uncultured bacterium | |
3.1.1.3 | Zn2+ | 162% activity at 0.1 mM | uncultured bacterium |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 43000 | - |
x * 43000, calculated from amino acid sequence | uncultured bacterium |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.1.1.3 | Acetone | the enzyme displays 100% activity in presence of 30% (v/v) acetone | uncultured bacterium |
3.1.1.3 | hexane | the enzyme displays 100% activity in presence of 30% (v/v) n-hexane | uncultured bacterium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.3 | uncultured bacterium | B6VGJ3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.3 | ammonium sulfate precipitation and phenyl Sepharose column chromatography | uncultured bacterium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 33 | - |
enzyme from supernatant, using 4-nitrophenyl laurate as substrate, at pH 8.0, 50°C | uncultured bacterium |
3.1.1.3 | 2022 | - |
enzyme after 61fold purification, using 4-nitrophenyl laurate as substrate, at pH 8.0, 50°C | uncultured bacterium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.3 | 4-nitrophenyl decanoate + H2O | about 25% activity compared to 4-nitrophenyl laurate | uncultured bacterium | 4-nitrophenol + decanoate | - |
? | |
3.1.1.3 | 4-nitrophenyl laurate + H2O | highest activity (100%) | uncultured bacterium | 4-nitrophenol + laurate | - |
? | |
3.1.1.3 | 4-nitrophenyl myristate + H2O | about 50% activity compared to 4-nitrophenyl laurate | uncultured bacterium | 4-nitrophenol + myristate | - |
? | |
3.1.1.3 | 4-nitrophenyl octanoate + H2O | about 3% activity compared to 4-nitrophenyl laurate | uncultured bacterium | 4-nitrophenol + octanoate | - |
? | |
3.1.1.3 | 4-nitrophenyl palmitate + H2O | about 18% activity compared to 4-nitrophenyl laurate | uncultured bacterium | 4-nitrophenol + palmitate | - |
? | |
3.1.1.3 | 4-nitrophenyl stearate + H2O | about 6% activity compared to 4-nitrophenyl laurate | uncultured bacterium | 4-nitrophenol + stearate | - |
? | |
3.1.1.3 | additional information | no activity with 4-nitrophenyl butyrate and 4-nitrophenyl propionate | uncultured bacterium | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.3 | ? | x * 43000, calculated from amino acid sequence | uncultured bacterium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.3 | lipase | - |
uncultured bacterium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 50 | - |
- |
uncultured bacterium |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 40 | 60 | the enzyme shows thermal stability up to 40°C without any loss of enzyme activity. Nearly 80% enzyme activity is retained at 50°C even after incubation for 75 min. However, above 50°C the enzyme displays thermal instability. At 55°C, enzyme activity almost reduces to 50, 44 and 32% after incubation for 45, 60 and 75 min, respectively. The half life of the enzyme is 5 min at 60°C | uncultured bacterium |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.3 | 569 | - |
4-nitrophenyl laurate | pH 8.0, 50°C | uncultured bacterium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 9 | - |
- |
uncultured bacterium |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 7 | 11 | the enzyme is completely stable at pH 8.0 and 9.0 when incubated at 22°C for 1 h, while nearly 50% enzyme activity is retained at pH 7.0, 10.0 and 11.0 | uncultured bacterium |