BRENDA - Enzyme Database

Enzymatic and biological characteristics of enolase in Brucella abortus A19

Han, X.; Ding, C.; Chen, H.; Hu, Q.; Yu, S.; Mol. Biol. Rep. 39, 2705-2711 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expression of His-tagged enolase in Escherichia coli strain BL21
Brucella abortus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
Cr2+
leads to complete inhibition at 10 mM
Brucella abortus
4.2.1.11
Cu2+
leads to complete inhibition at 10 mM
Brucella abortus
4.2.1.11
Hg2+
leads to complete inhibition at 10 mM
Brucella abortus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
2
-
2-phospho-D-glycerate
pH 8.5, 25°C, recombinant enzyme
Brucella abortus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Al3+
can partially substitute for Mg2+ or Zn2+ at 10 mM showing 68.8% of the maximal activity with 10 mM Mg2+
Brucella abortus
4.2.1.11
Mg2+
activates, optimally at 10 mM
Brucella abortus
4.2.1.11
Mn2+
can partially substitute for Mg2+ or Zn2+ at 10 mM showing 90.4% of the maximal activity with 10 mM Mg2+
Brucella abortus
4.2.1.11
additional information
57% of maximal activity without metal ion compared to Mg2+ at 10 mM
Brucella abortus
4.2.1.11
Ni2+
can partially substitute for Mg2+ or Zn2+ at 10 mM showing 90.5% of the maximal activity with 10 mM Mg2+
Brucella abortus
4.2.1.11
Zn2+
activates, optimally at 10 mM, best activating metal ion
Brucella abortus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
49000
-
x * 49000, recombinant His-tagged enolase, SDS-PAGE
Brucella abortus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Brucella abortus
-
phosphoenolpyruvate + H2O
-
-
?
4.2.1.11
2-phospho-D-glycerate
Brucella abortus A19
-
phosphoenolpyruvate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Brucella abortus
Q2YPV0
strain 2308, 100% sequence identity with strain A19
-
4.2.1.11
Brucella abortus A19
Q2YPV0
strain 2308, 100% sequence identity with strain A19
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
recombinant His-tagged enolase from Escherichia coli strain BL21 by nickel affinity chromatography
Brucella abortus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
3909
-
purifed recombinant enzyme, pH 8.5, 37°C, no metal ion added
Brucella abortus
4.2.1.11
9091
-
purifed recombinant enzyme, pH 8.5, 37°C, 10 mM Mg2+
Brucella abortus
4.2.1.11
10050
-
purifed recombinant enzyme, pH 8.5, 37°C, 10 mM Zn2+
Brucella abortus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
716180
Brucella abortus
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
716180
Brucella abortus A19
phosphoenolpyruvate + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 49000, recombinant His-tagged enolase, SDS-PAGE
Brucella abortus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.11
37
-
recombinant enzyme
Brucella abortus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.11
25
62
maximally stable at 37°C, the purified recombinant enolase is thermally stable at 25°C, 37°C, 50°C, and 62°C, but is completely inhibited by a preincubation of the enzyme at 75°C after 1 h
Brucella abortus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
8.5
-
recombinant enzyme
Brucella abortus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.11
5
10
activity range, recombinant enzyme
Brucella abortus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expression of His-tagged enolase in Escherichia coli strain BL21
Brucella abortus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
Cr2+
leads to complete inhibition at 10 mM
Brucella abortus
4.2.1.11
Cu2+
leads to complete inhibition at 10 mM
Brucella abortus
4.2.1.11
Hg2+
leads to complete inhibition at 10 mM
Brucella abortus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
2
-
2-phospho-D-glycerate
pH 8.5, 25°C, recombinant enzyme
Brucella abortus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Al3+
can partially substitute for Mg2+ or Zn2+ at 10 mM showing 68.8% of the maximal activity with 10 mM Mg2+
Brucella abortus
4.2.1.11
Mg2+
activates, optimally at 10 mM
Brucella abortus
4.2.1.11
Mn2+
can partially substitute for Mg2+ or Zn2+ at 10 mM showing 90.4% of the maximal activity with 10 mM Mg2+
Brucella abortus
4.2.1.11
additional information
57% of maximal activity without metal ion compared to Mg2+ at 10 mM
Brucella abortus
4.2.1.11
Ni2+
can partially substitute for Mg2+ or Zn2+ at 10 mM showing 90.5% of the maximal activity with 10 mM Mg2+
Brucella abortus
4.2.1.11
Zn2+
activates, optimally at 10 mM, best activating metal ion
Brucella abortus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
49000
-
x * 49000, recombinant His-tagged enolase, SDS-PAGE
Brucella abortus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Brucella abortus
-
phosphoenolpyruvate + H2O
-
-
?
4.2.1.11
2-phospho-D-glycerate
Brucella abortus A19
-
phosphoenolpyruvate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
recombinant His-tagged enolase from Escherichia coli strain BL21 by nickel affinity chromatography
Brucella abortus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
3909
-
purifed recombinant enzyme, pH 8.5, 37°C, no metal ion added
Brucella abortus
4.2.1.11
9091
-
purifed recombinant enzyme, pH 8.5, 37°C, 10 mM Mg2+
Brucella abortus
4.2.1.11
10050
-
purifed recombinant enzyme, pH 8.5, 37°C, 10 mM Zn2+
Brucella abortus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
716180
Brucella abortus
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
716180
Brucella abortus A19
phosphoenolpyruvate + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 49000, recombinant His-tagged enolase, SDS-PAGE
Brucella abortus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.11
37
-
recombinant enzyme
Brucella abortus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.11
25
62
maximally stable at 37°C, the purified recombinant enolase is thermally stable at 25°C, 37°C, 50°C, and 62°C, but is completely inhibited by a preincubation of the enzyme at 75°C after 1 h
Brucella abortus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
8.5
-
recombinant enzyme
Brucella abortus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.11
5
10
activity range, recombinant enzyme
Brucella abortus
General Information
EC Number
General Information
Commentary
Organism
4.2.1.11
physiological function
the recombinant enolase exhibits fibronectin-binding ability in immunoblotting assay, suggesting that enolase may play a role in Brucella abortus colonization, persistence, and invasion of host tissue
Brucella abortus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.2.1.11
physiological function
the recombinant enolase exhibits fibronectin-binding ability in immunoblotting assay, suggesting that enolase may play a role in Brucella abortus colonization, persistence, and invasion of host tissue
Brucella abortus