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Literature summary extracted from

  • Hu, G.; Jia, J.; Hur, Y.; Chung, Y.; Lee, J.; Yun, D.; Chung, W.; Yi, G.; Kim, T.; Kim, D.
    Molecular characterization of phenylalanine ammonia lyase gene from Cistanche deserticola (2010), Mol. Biol. Rep., 38, 3741-3750.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.24 expression in Escherichia coli Cistanche deserticola

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.24 2-aminoindan-2-phosphonic acid competitive, specific inhibitor Cistanche deserticola
4.3.1.24 chlorogenic acid uncompetitive Cistanche deserticola
4.3.1.24 Cinnamic acid mixed type Cistanche deserticola
4.3.1.24 cinnamyl alcohol uncompetitive Cistanche deserticola
4.3.1.24 coniferyl alcohol competitive Cistanche deserticola
4.3.1.24 coniferyl aldehyde competitive Cistanche deserticola
4.3.1.24 coumaric acid uncompetitive Cistanche deserticola
4.3.1.24 Hg2+ 1 mM, 65% loss of activity Cistanche deserticola
4.3.1.24 additional information not inhibitory: tyrosol, caffeic acid Cistanche deserticola
4.3.1.24 Pb2+ 1 mM, 50% loss of activity Cistanche deserticola
4.3.1.24 salidroside competitive Cistanche deserticola
4.3.1.24 Zn2+ 1 mM, 65% loss of activity Cistanche deserticola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.24 0.101
-
 L-Phe pH 8.5, 55°C Cistanche deserticola

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.24 Cs+ 1 mM, weak enhancement Cistanche deserticola
4.3.1.24 Mn2+ 1 mM, weak enhancement Cistanche deserticola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.24 94000
-
 x * 94000, SDS-PAGE of recombinant protein with GST-tag Cistanche deserticola

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.24 Cistanche deserticola D3Y5L6 isoform PAL1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.24 L-Phe
-
 Cistanche deserticola (E)-cinnamate + NH3
-
 ?
4.3.1.24 additional information no substrate: L-Tyr Cistanche deserticola ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.24 ? x * 94000, SDS-PAGE of recombinant protein with GST-tag Cistanche deserticola

Synonyms

EC Number Synonyms Comment Organism
4.3.1.24 PAL1
-
 Cistanche deserticola

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.24 55
-
-
 Cistanche deserticola

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.1.24 70
-
 20 min, retains more than 85% of activity Cistanche deserticola
4.3.1.24 75
-
 20 min, loss of more than 85% of activity Cistanche deserticola

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.1.24 3.36
-
 L-Phe pH 8.5, 55°C Cistanche deserticola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.24 8.5
-
-
 Cistanche deserticola

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.3.1.24 0.000056
-
 2-aminoindan-2-phosphonic acid pH 8.5, 55°C Cistanche deserticola
4.3.1.24 0.008
-
 Cinnamic acid pH 8.5, 55°C Cistanche deserticola
4.3.1.24 0.013
-
 coniferyl aldehyde pH 8.5, 55°C Cistanche deserticola
4.3.1.24 0.017
-
 salidroside pH 8.5, 55°C Cistanche deserticola
4.3.1.24 0.032
-
 coniferyl alcohol pH 8.5, 55°C Cistanche deserticola
4.3.1.24 0.032
-
 coumaric acid pH 8.5, 55°C Cistanche deserticola
4.3.1.24 0.037
-
 cinnamyl alcohol pH 8.5, 55°C Cistanche deserticola
4.3.1.24 0.043
-
 chlorogenic acid pH 8.5, 55°C Cistanche deserticola

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3.1.24 33.17
-
 L-Phe pH 8.5, 55°C Cistanche deserticola