BRENDA - Enzyme Database

Clonorchis sinensis enolase: identification and biochemical characterization of a glycolytic enzyme from excretory/secretory products

Wang, X.; Chen, W.; Hu, F.; Deng, C.; Zhou, C.; Lv, X.; Fan, Y.; Men, J.; Huang, Y.; Sun, J.; Hu, D.; Chen, J.; Yang, Y.; Liang, C.; Zheng, H.; Hu, X.; Xu, J.; Wu, Z.; Yu, X.; Mol. Biochem. Parasitol. 177, 135-142 (2011)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
additional information
enolase as both an excretory/secretory product and a tegumental component of Clonorchis sinensis, immunohistochemical analysis
Clonorchis sinensis
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
K+
activates, best at 2 mM
Clonorchis sinensis
4.2.1.11
Mg2+
activates, best at 10 mM
Clonorchis sinensis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Clonorchis sinensis
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
additional information
Clonorchis sinensis
enolase shows plasminogen-binding activity
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Clonorchis sinensis
-
propagated in livers of male Sprague-Dawley rats
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
integument
adult worm
Clonorchis sinensis
-
4.2.1.11
additional information
enolase expression analysis in adult worm, metacercaria, cercaria and egg. Csenolase is transcribed at the four life stages of Clonorchis sinensis while showing a significant higher expression level at the stage of adult worm
Clonorchis sinensis
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
716155
Clonorchis sinensis
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
additional information
enolase shows plasminogen-binding activity
716155
Clonorchis sinensis
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.11
37
-
assay at
Clonorchis sinensis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7
8
-
Clonorchis sinensis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
additional information
enolase as both an excretory/secretory product and a tegumental component of Clonorchis sinensis, immunohistochemical analysis
Clonorchis sinensis
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
K+
activates, best at 2 mM
Clonorchis sinensis
4.2.1.11
Mg2+
activates, best at 10 mM
Clonorchis sinensis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Clonorchis sinensis
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
additional information
Clonorchis sinensis
enolase shows plasminogen-binding activity
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
integument
adult worm
Clonorchis sinensis
-
4.2.1.11
additional information
enolase expression analysis in adult worm, metacercaria, cercaria and egg. Csenolase is transcribed at the four life stages of Clonorchis sinensis while showing a significant higher expression level at the stage of adult worm
Clonorchis sinensis
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
716155
Clonorchis sinensis
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
additional information
enolase shows plasminogen-binding activity
716155
Clonorchis sinensis
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.11
37
-
assay at
Clonorchis sinensis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7
8
-
Clonorchis sinensis
General Information
EC Number
General Information
Commentary
Organism
4.2.1.11
physiological function
enolase is a multifunctional enzyme that is involved in the reversible dehydration of 2-phospho-D-glycerate to phosphoenolpyruvate in glycolytic and gluconeogenesis pathways. Csenolase might play key roles in the growth of the parasites. Csenolase is an important glycolytic enzyme required for the development of Clonorchis sinensis
Clonorchis sinensis
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.2.1.11
physiological function
enolase is a multifunctional enzyme that is involved in the reversible dehydration of 2-phospho-D-glycerate to phosphoenolpyruvate in glycolytic and gluconeogenesis pathways. Csenolase might play key roles in the growth of the parasites. Csenolase is an important glycolytic enzyme required for the development of Clonorchis sinensis
Clonorchis sinensis