BRENDA - Enzyme Database

The dddP gene of Roseovarius nubinhibens encodes a novel lyase that cleaves dimethylsulfoniopropionate into acrylate plus dimethyl sulfide

Kirkwood, M.; Le Brun, N.E.; Todd, J.D.; Johnston, A.W.; Microbiology 156, 1900-1906 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.4.1.3
expression in Escherichia coli
Roseovarius nubinhibens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.4.1.3
D295A
complete loss of activity
Roseovarius nubinhibens
4.4.1.3
D297A
complete loss of activity
Roseovarius nubinhibens
4.4.1.3
D307A
complete loss of activity
Roseovarius nubinhibens
4.4.1.3
E406A
complete loss of activity
Roseovarius nubinhibens
4.4.1.3
E421A
complete loss of activity
Roseovarius nubinhibens
4.4.1.3
H371A
complete loss of activity
Roseovarius nubinhibens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.4.1.3
additional information
not inhibitory: EDTA at 25 mM, bipyridyl at 2.5 mM
Roseovarius nubinhibens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.4.1.3
14
-
beta-dimethylsulfoniopropanoate
pH 6.0, 30C
Roseovarius nubinhibens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.4.1.3
additional information
none of the metals Co, Cu, Mn, Ni or Zn is detected at above background levels
Roseovarius nubinhibens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.4.1.3
49972
-
2 * 49972, calculated, 2 * 50000, SDS-PAGE
Roseovarius nubinhibens
4.4.1.3
50000
-
2 * 49972, calculated, 2 * 50000, SDS-PAGE
Roseovarius nubinhibens
4.4.1.3
95300
-
sedimentation equilibrium ultracentrifugation
Roseovarius nubinhibens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.4.1.3
Roseovarius nubinhibens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.4.1.3
recombinant protein
Roseovarius nubinhibens
Storage Stability
EC Number
Storage Stability
Organism
4.4.1.3
4C, 50 mM MES, pH 6, no detectable loss of activity over a period of several weeks
Roseovarius nubinhibens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.4.1.3
beta-dimethylsulfoniopropanoate
-
716137
Roseovarius nubinhibens
dimethylsulfide + acrylate
-
-
-
?
4.4.1.3
additional information
enzyme is member of the M24B family, which acts on a non-peptide substrate and has no metal cofactor
716137
Roseovarius nubinhibens
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.4.1.3
dimer
2 * 49972, calculated, 2 * 50000, SDS-PAGE
Roseovarius nubinhibens
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.4.1.3
30
-
assay at
Roseovarius nubinhibens
4.4.1.3
55
60
-
Roseovarius nubinhibens
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.4.1.3
60
-
rapid loss of activity above
Roseovarius nubinhibens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.4.1.3
6
-
-
Roseovarius nubinhibens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.4.1.3
5
7
more than 70% of maximum activity
Roseovarius nubinhibens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.4.1.3
expression in Escherichia coli
Roseovarius nubinhibens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.4.1.3
D295A
complete loss of activity
Roseovarius nubinhibens
4.4.1.3
D297A
complete loss of activity
Roseovarius nubinhibens
4.4.1.3
D307A
complete loss of activity
Roseovarius nubinhibens
4.4.1.3
E406A
complete loss of activity
Roseovarius nubinhibens
4.4.1.3
E421A
complete loss of activity
Roseovarius nubinhibens
4.4.1.3
H371A
complete loss of activity
Roseovarius nubinhibens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.4.1.3
additional information
not inhibitory: EDTA at 25 mM, bipyridyl at 2.5 mM
Roseovarius nubinhibens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.4.1.3
14
-
beta-dimethylsulfoniopropanoate
pH 6.0, 30C
Roseovarius nubinhibens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.4.1.3
additional information
none of the metals Co, Cu, Mn, Ni or Zn is detected at above background levels
Roseovarius nubinhibens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.4.1.3
49972
-
2 * 49972, calculated, 2 * 50000, SDS-PAGE
Roseovarius nubinhibens
4.4.1.3
50000
-
2 * 49972, calculated, 2 * 50000, SDS-PAGE
Roseovarius nubinhibens
4.4.1.3
95300
-
sedimentation equilibrium ultracentrifugation
Roseovarius nubinhibens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.4.1.3
recombinant protein
Roseovarius nubinhibens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.4.1.3
4C, 50 mM MES, pH 6, no detectable loss of activity over a period of several weeks
Roseovarius nubinhibens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.4.1.3
beta-dimethylsulfoniopropanoate
-
716137
Roseovarius nubinhibens
dimethylsulfide + acrylate
-
-
-
?
4.4.1.3
additional information
enzyme is member of the M24B family, which acts on a non-peptide substrate and has no metal cofactor
716137
Roseovarius nubinhibens
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.4.1.3
dimer
2 * 49972, calculated, 2 * 50000, SDS-PAGE
Roseovarius nubinhibens
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.4.1.3
30
-
assay at
Roseovarius nubinhibens
4.4.1.3
55
60
-
Roseovarius nubinhibens
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.4.1.3
60
-
rapid loss of activity above
Roseovarius nubinhibens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.4.1.3
6
-
-
Roseovarius nubinhibens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.4.1.3
5
7
more than 70% of maximum activity
Roseovarius nubinhibens