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Literature summary extracted from

  • Takamatsu, S.; Lin, X.; Nara, A.; Komatsu, M.; Cane, D.E.; Ikeda, H.
    Characterization of a silent sesquiterpenoid biosynthetic pathway in Streptomyces avermitilis controlling epi-isozizaene albaflavenone biosynthesis and isolation of a new oxidized epi-isozizaene metabolite (2011), Microb. Biotechnol., 4, 184-191.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.37 expression in Escherichia coli Streptomyces avermitilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.37 0.000125
-
 (2E,6E)-farnesyl diphosphate pH 7.0, 30°C Streptomyces avermitilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.37 Mg2+ required Streptomyces avermitilis
4.2.3.37 Mn2+ 32% of the activity with Mg2+ Streptomyces avermitilis
4.2.3.37 Zn2+ 5% of the activity with Mg2+ Streptomyces avermitilis

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.37 Streptomyces avermitilis Q82IV1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.37 (2E,6E)-farnesyl diphosphate
-
 Streptomyces avermitilis (+)-epi-isozizaene + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.37 SAV_3032
-
 Streptomyces avermitilis
4.2.3.37 TPS2
-
 Streptomyces avermitilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.37 30
-
 assay at Streptomyces avermitilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.37 0.00026
-
 (2E,6E)-farnesyl diphosphate pH 7.0, 30°C Streptomyces avermitilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.37 7
-
-
 Streptomyces avermitilis

General Information

EC Number General Information Comment Organism
4.2.3.37 physiological function Streptomyces avermitilis does not produce epi-isozizaene or any of its oxidized derivatives, albaflavenols and albaflavenone, in any culture conditions examined. Recombinant SAV_3032 protein expressed in Escherichia coli catalyses the Mg2+-dependent cyclization of farnesyl diphosphate to epi-isozizaene Streptomyces avermitilis