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Literature summary extracted from

  • Sedgwick, B.; Robins, P.; Lindahl, T.
    Direct removal of alkylation damage from DNA by AlkB and related DNA dioxygenases (2006), Methods Enzymol., 408, 108-120.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.33 expressed in Escherichia coli BL21(DE3) cells Escherichia coli
1.14.11.33 expressed in Escherichia coli BL21(DE3) cells and in Sf9 insect cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.33 nucleus
-
 Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.33 Fe2+ required for activity Homo sapiens
1.14.11.33 Fe2+ required for activity Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.33 Escherichia coli P05050
-
-
1.14.11.33 Homo sapiens Q6NS38 isoform ABH2
-
1.14.11.33 Homo sapiens Q96Q83 isoform ABH3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.33 His-tagged AlkB is purified by Ni-NTA agarose column chromatography, untagged Alkb is purified by DEAE-cellulose column chromatography, Sepharose column chromatography, and Mono S column chromatography Escherichia coli
1.14.11.33 His-tagged enzyme is purified by Ni-NTA agarose column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.33 DNA-1-ethyladenine + 2-oxoglutarate + O2
-
 Homo sapiens DNA-adenine + acetaldehyde + succinate + CO2
-
 ir
1.14.11.33 DNA-1-ethyladenine + 2-oxoglutarate + O2
-
 Escherichia coli DNA-adenine + acetaldehyde + succinate + CO2
-
 ir
1.14.11.33 DNA-1-methyladenine + 2-oxoglutarate + O2 good substrate Homo sapiens DNA-adenine + formaldehyde + succinate + CO2
-
 ir
1.14.11.33 DNA-1-methyladenine + 2-oxoglutarate + O2 good substrate Escherichia coli DNA-adenine + formaldehyde + succinate + CO2
-
 ir
1.14.11.33 DNA-1-methylguanine + 2-oxoglutarate + O2 weak substrate Homo sapiens DNA-guanine + formaldehyde + succinate + CO2
-
 ir
1.14.11.33 DNA-1-methylguanine + 2-oxoglutarate + O2 weak substrate Escherichia coli DNA-guanine + formaldehyde + succinate + CO2
-
 ir
1.14.11.33 DNA-3-methylcytosine + 2-oxoglutarate + O2 good substrate Homo sapiens DNA-cytosine + formaldehyde + succinate + CO2
-
 ir
1.14.11.33 DNA-3-methylcytosine + 2-oxoglutarate + O2 good substrate Escherichia coli DNA-cytosine + formaldehyde + succinate + CO2
-
 ir
1.14.11.33 DNA-3-methylthymine + 2-oxoglutarate + O2 weak substrate Homo sapiens DNA-thymine + formaldehyde + succinate + CO2
-
 ir
1.14.11.33 DNA-3-methylthymine + 2-oxoglutarate + O2 weak substrate Escherichia coli DNA-thymine + formaldehyde + succinate + CO2
-
 ir
1.14.11.33 methylated poly(deoxyadenine) + 2-oxoglutarate + O2
-
 Homo sapiens poly(deoxyadenine) + formaldehyde + succinate + CO2
-
 ir
1.14.11.33 methylated poly(deoxyadenine) + 2-oxoglutarate + O2
-
 Escherichia coli poly(deoxyadenine) + formaldehyde + succinate + CO2
-
 ir
1.14.11.33 methylated poly(deoxycytosine) + 2-oxoglutarate + O2
-
 Homo sapiens poly(deoxycytosine) + formaldehyde + succinate + CO2
-
 ir
1.14.11.33 methylated poly(deoxycytosine) + 2-oxoglutarate + O2
-
 Escherichia coli poly(deoxycytosine) + formaldehyde + succinate + CO2
-
 ir
1.14.11.33 additional information AlkB binds to single-stranded DNA, but less efficiently to double-stranded DNA Escherichia coli ?
-
 ?
1.14.11.33 additional information isoform ABH2 shows some preference for methylated poly(dA) over methylated poly(dC) and also prefers a double-stranded substrate. not ABH2 cannot demethylate 1-methyladenine in RNA Homo sapiens ?
-
 ?
1.14.11.33 additional information isoform ABH2 shows some preference for methylated poly(dC) over methylated poly(dA) and also prefers a single-stranded substrate. Isoform ABH3 can also demethylate 1-methyladenine in RNA Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.33 ABH2 isoform Homo sapiens
1.14.11.33 ABH3 isoform Homo sapiens
1.14.11.33 AlkB
-
 Homo sapiens
1.14.11.33 AlkB
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.33 2-oxoglutarate
-
 Homo sapiens
1.14.11.33 2-oxoglutarate
-
 Escherichia coli