BRENDA - Enzyme Database

Fe-only hydrogenase from Thermotoga maritima

Verhagen, M.; Adams, M.; Methods Enzymol. 331, 216-226 (2001) View publication on PubMed

Data extracted from this reference:

Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.12.1.4
Fe2+
the holoprotein contains 31.1 mol iron per mol enzyme
Thermotoga maritima
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.12.1.4
18025
-
1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence
Thermotoga maritima
1.12.1.4
19000
-
1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE
Thermotoga maritima
1.12.1.4
68000
-
1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE
Thermotoga maritima
1.12.1.4
68676
-
1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence
Thermotoga maritima
1.12.1.4
72248
-
1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence
Thermotoga maritima
1.12.1.4
73000
-
1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE
Thermotoga maritima
1.12.1.4
158900
-
gel filtration
Thermotoga maritima
1.12.1.4
160000
-
calculated from amino acid sequence
Thermotoga maritima
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.12.1.4
NADH + H+ + reduced ferredoxin
Thermotoga maritima
-
H2 + NAD+ + oxidized ferredoxin
-
-
?
1.12.1.4
NADH + H+ + reduced ferredoxin
Thermotoga maritima DSM 3109
-
H2 + NAD+ + oxidized ferredoxin
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.12.1.4
Thermotoga maritima
-
-
-
1.12.1.4
Thermotoga maritima DSM 3109
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.12.1.4
DEAE-Sepharose column chromatography, Q-Sepharose column chromatography, hydroxyapatite column chromatography, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration
Thermotoga maritima
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.12.1.4
NADH + H+ + reduced ferredoxin
-
716106
Thermotoga maritima
H2 + NAD+ + oxidized ferredoxin
-
-
-
?
1.12.1.4
NADH + H+ + reduced ferredoxin
-
716106
Thermotoga maritima DSM 3109
H2 + NAD+ + oxidized ferredoxin
-
-
-
?
1.12.1.4
sodium dithionite + H+ + reduced anthraquinone-2,6-disulfonic acid
-
716106
Thermotoga maritima
H2 + oxidized anthraquinone-2,6-disulfonic acid + ?
-
-
-
?
1.12.1.4
sodium dithionite + H+ + reduced anthraquinone-2,6-disulfonic acid
-
716106
Thermotoga maritima DSM 3109
H2 + oxidized anthraquinone-2,6-disulfonic acid + ?
-
-
-
?
1.12.1.4
sodium dithionite + H+ + reduced methyl viologen
-
716106
Thermotoga maritima
H2 + oxidized methyl viologen + ?
-
-
-
?
1.12.1.4
sodium dithionite + H+ + reduced methyl viologen
-
716106
Thermotoga maritima DSM 3109
H2 + oxidized methyl viologen + ?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.12.1.4
heterotrimer
1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence
Thermotoga maritima
1.12.1.4
heterotrimer
1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE
Thermotoga maritima
Synonyms
EC Number
Synonyms
Commentary
Organism
1.12.1.4
Fe-only hydrogenase
-
Thermotoga maritima
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.12.1.4
flavin
-
Thermotoga maritima
1.12.1.4
iron-sulfur centre
the alpha subunit contains two [2Fe-2S] clusters and three [4Fe-4S] clusters, the beta subunit contains three [4Fe-4S] clusters and one [2Fe-2S] clusters, the gamma subunit contains one [2Fe-2S] cluster
Thermotoga maritima
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.12.1.4
flavin
-
Thermotoga maritima
1.12.1.4
iron-sulfur centre
the alpha subunit contains two [2Fe-2S] clusters and three [4Fe-4S] clusters, the beta subunit contains three [4Fe-4S] clusters and one [2Fe-2S] clusters, the gamma subunit contains one [2Fe-2S] cluster
Thermotoga maritima
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.12.1.4
Fe2+
the holoprotein contains 31.1 mol iron per mol enzyme
Thermotoga maritima
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.12.1.4
18025
-
1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence
Thermotoga maritima
1.12.1.4
19000
-
1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE
Thermotoga maritima
1.12.1.4
68000
-
1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE
Thermotoga maritima
1.12.1.4
68676
-
1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence
Thermotoga maritima
1.12.1.4
72248
-
1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence
Thermotoga maritima
1.12.1.4
73000
-
1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE
Thermotoga maritima
1.12.1.4
158900
-
gel filtration
Thermotoga maritima
1.12.1.4
160000
-
calculated from amino acid sequence
Thermotoga maritima
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.12.1.4
NADH + H+ + reduced ferredoxin
Thermotoga maritima
-
H2 + NAD+ + oxidized ferredoxin
-
-
?
1.12.1.4
NADH + H+ + reduced ferredoxin
Thermotoga maritima DSM 3109
-
H2 + NAD+ + oxidized ferredoxin
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.12.1.4
DEAE-Sepharose column chromatography, Q-Sepharose column chromatography, hydroxyapatite column chromatography, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration
Thermotoga maritima
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.12.1.4
NADH + H+ + reduced ferredoxin
-
716106
Thermotoga maritima
H2 + NAD+ + oxidized ferredoxin
-
-
-
?
1.12.1.4
NADH + H+ + reduced ferredoxin
-
716106
Thermotoga maritima DSM 3109
H2 + NAD+ + oxidized ferredoxin
-
-
-
?
1.12.1.4
sodium dithionite + H+ + reduced anthraquinone-2,6-disulfonic acid
-
716106
Thermotoga maritima
H2 + oxidized anthraquinone-2,6-disulfonic acid + ?
-
-
-
?
1.12.1.4
sodium dithionite + H+ + reduced anthraquinone-2,6-disulfonic acid
-
716106
Thermotoga maritima DSM 3109
H2 + oxidized anthraquinone-2,6-disulfonic acid + ?
-
-
-
?
1.12.1.4
sodium dithionite + H+ + reduced methyl viologen
-
716106
Thermotoga maritima
H2 + oxidized methyl viologen + ?
-
-
-
?
1.12.1.4
sodium dithionite + H+ + reduced methyl viologen
-
716106
Thermotoga maritima DSM 3109
H2 + oxidized methyl viologen + ?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.12.1.4
heterotrimer
1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence
Thermotoga maritima
1.12.1.4
heterotrimer
1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE
Thermotoga maritima