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Literature summary extracted from

  • Yamanaka, T.
    Sulfide-cytochrome c reductase (flavocytochrome c) (1994), Methods Enzymol., 243, 463-472.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.2.3 cyanide
-
 Allochromatium vinosum
1.8.2.3 cyanide
-
 Chlorobaculum thiosulfatiphilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.2.3 11000
-
 1 * 47000 + 1 * 11000, flavoprotein subunit and cytochrome subunit, SDS-PAGE Chlorobaculum thiosulfatiphilum
1.8.2.3 21000
-
 1 * 46000 + 1 * 21000, flavoprotein subunit and cytochrome subunit, SDS-PAGE Allochromatium vinosum
1.8.2.3 46000
-
 1 * 46000 + 1 * 21000, flavoprotein subunit and cytochrome subunit, SDS-PAGE Allochromatium vinosum
1.8.2.3 47000
-
 1 * 47000 + 1 * 11000, flavoprotein subunit and cytochrome subunit, SDS-PAGE Chlorobaculum thiosulfatiphilum
1.8.2.3 58000
-
 gel filtration Chlorobaculum thiosulfatiphilum
1.8.2.3 72000
-
 gel filtration Allochromatium vinosum

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.3 Allochromatium vinosum
-
-
-
1.8.2.3 Chlorobaculum thiosulfatiphilum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.2.3 ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Amberlite CG-50 column chromatography Allochromatium vinosum
1.8.2.3 ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Amberlite CG-50 column chromatography Chlorobaculum thiosulfatiphilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.3 sulfide + oxidized benzyl viologen
-
 Allochromatium vinosum sulfur + reduced benzyl viologen + H+
-
 ?
1.8.2.3 sulfide + oxidized benzyl viologen
-
 Chlorobaculum thiosulfatiphilum sulfur + reduced benzyl viologen + H+
-
 ?
1.8.2.3 sulfide + oxidized flavocytochrome c
-
 Allochromatium vinosum sulfur + reduced flavocytochrome c + H+
-
 ?
1.8.2.3 sulfide + oxidized flavocytochrome c
-
 Chlorobaculum thiosulfatiphilum sulfur + reduced flavocytochrome c + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.2.3 heterodimer 1 * 46000 + 1 * 21000, flavoprotein subunit and cytochrome subunit, SDS-PAGE Allochromatium vinosum
1.8.2.3 heterodimer 1 * 47000 + 1 * 11000, flavoprotein subunit and cytochrome subunit, SDS-PAGE Chlorobaculum thiosulfatiphilum

Synonyms

EC Number Synonyms Comment Organism
1.8.2.3 sulfide-cytochrome c reductase (flavocytochrome c)
-
 Allochromatium vinosum
1.8.2.3 sulfide-cytochrome c reductase (flavocytochrome c)
-
 Chlorobaculum thiosulfatiphilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.2.3 FAD covalently bound, the enzyme contains one molecule of FAD per molecule Allochromatium vinosum
1.8.2.3 FAD covalently bound, the enzyme contains one molecule of FAD per molecule Chlorobaculum thiosulfatiphilum
1.8.2.3 heme covalently bound heme c, the enzyme contains one molecule of heme c per molecule Chlorobaculum thiosulfatiphilum
1.8.2.3 heme covalently bound heme c, the enzyme contains two molecules of heme c per molecule Allochromatium vinosum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.8.2.3 Allochromatium vinosum isoelectric focusing
-
 5.1
1.8.2.3 Chlorobaculum thiosulfatiphilum isoelectric focusing
-
 6.7