BRENDA - Enzyme Database

Engineering Escherichia coli for efficient production of 5-aminolevulinic acid from glucose

Kang, Z.; Wang, Y.; Gu, P.; Wang, Q.; Qi, Q.; Metab. Eng. 13, 492-498 (2011)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
1.2.1.70
biotechnology
recombinant Escherichia coli allows efficient production of 5-aminolevulinic acid directly from glucose
Salmonella enterica subsp. arizonae
Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.70
hemA ligated into pUC19 vector with a Lys insert between Thr-2 and Leu-3 at N terminus, overexpression in Escherichia coli
Salmonella enterica subsp. arizonae
5.4.3.8
expressed in Escherichia coli DEX cells
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.70
Salmonella enterica subsp. arizonae
-
-
-
5.4.3.8
Escherichia coli
-
-
-
5.4.3.8
Escherichia coli MG1655
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
-
716103
Salmonella enterica subsp. arizonae
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
-
1.2.1.70
additional information
hemA with Lys insertion overexpressing Escherichia coli strain shows an increased 5-aminolevulinic acid accumulation indicating that the reduction of glutamyl-tRNA to glutamate-1-semialdehyde is a rate-limiting step
716103
Salmonella enterica subsp. arizonae
?
-
-
-
?
5.4.3.8
L-glutamate 1-semialdehyde
-
716103
Escherichia coli
5-aminolevulinate
-
-
-
?
5.4.3.8
L-glutamate 1-semialdehyde
-
716103
Escherichia coli MG1655
5-aminolevulinate
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.3.8
pyridoxal 5'-phosphate
dependent on
Escherichia coli
Application (protein specific)
EC Number
Application
Commentary
Organism
1.2.1.70
biotechnology
recombinant Escherichia coli allows efficient production of 5-aminolevulinic acid directly from glucose
Salmonella enterica subsp. arizonae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.70
hemA ligated into pUC19 vector with a Lys insert between Thr-2 and Leu-3 at N terminus, overexpression in Escherichia coli
Salmonella enterica subsp. arizonae
5.4.3.8
expressed in Escherichia coli DEX cells
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.3.8
pyridoxal 5'-phosphate
dependent on
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
-
716103
Salmonella enterica subsp. arizonae
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
-
1.2.1.70
additional information
hemA with Lys insertion overexpressing Escherichia coli strain shows an increased 5-aminolevulinic acid accumulation indicating that the reduction of glutamyl-tRNA to glutamate-1-semialdehyde is a rate-limiting step
716103
Salmonella enterica subsp. arizonae
?
-
-
-
?
5.4.3.8
L-glutamate 1-semialdehyde
-
716103
Escherichia coli
5-aminolevulinate
-
-
-
?
5.4.3.8
L-glutamate 1-semialdehyde
-
716103
Escherichia coli MG1655
5-aminolevulinate
-
-
-
?
Expression
EC Number
Organism
Commentary
Expression
5.4.3.8
Escherichia coli
overexpression of gltX does not affect the transcription of hemL
additional information
General Information
EC Number
General Information
Commentary
Organism
5.4.3.8
physiological function
co-expression of hemL with hemAM achieves asignificantly increased 5-aminolevulinate production
Escherichia coli
General Information (protein specific)
EC Number
General Information
Commentary
Organism
5.4.3.8
physiological function
co-expression of hemL with hemAM achieves asignificantly increased 5-aminolevulinate production
Escherichia coli
Expression (protein specific)
EC Number
Organism
Commentary
Expression
5.4.3.8
Escherichia coli
overexpression of gltX does not affect the transcription of hemL
additional information