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Literature summary extracted from

  • Park, H.; Kam, N.; Lee, E.; Kim, H.
    Cloning and characterization of a novel oligoalginate lyase from a newly isolated bacterium Sphingomonas sp. MJ-3 (2012), Mar. Biotechnol., 14, 189-202.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.2.3 synthesis the recombinant MJ-3 alginate lyase can be used as a biocatalyst for saccharification of alginate since it can efficiently degrade poly-M block, poly-G block, poly-MG block, alginate oligosaccharides, and alginate into alginate monosaccharides Sphingomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Sphingomonas sp.
4.2.2.3 expression in Escherichia coli Sphingomonas sp.
4.2.2.11 expression in Escherichia coli Sphingomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 78000
-
 x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE Sphingomonas sp.
4.2.2.3 79600
-
 x * 79600, recombinant His6-tagged enzyme, SDS-PAGE Sphingomonas sp.
4.2.2.3 79900
-
 x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE Sphingomonas sp.
4.2.2.3 80000
-
 x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE Sphingomonas sp.
4.2.2.11 78000
-
 x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE Sphingomonas sp.
4.2.2.11 79900
-
 x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE Sphingomonas sp.
4.2.2.11 80000
-
 x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE Sphingomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Sphingomonas sp. G1EH67
-
-
4.2.2.3 Sphingomonas sp. G1EH67 isolated from fermented brown seaweed with salted and fermented anchovy, sea mustard, Undaria pinnatifida
-
4.2.2.3 Sphingomonas sp. MJ-3 G1EH67
-
-
4.2.2.3 Sphingomonas sp. MJ-3 G1EH67 isolated from fermented brown seaweed with salted and fermented anchovy, sea mustard, Undaria pinnatifida
-
4.2.2.11 Sphingomonas sp. G1EH67
-
-
4.2.2.11 Sphingomonas sp. MJ-3 G1EH67
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Sphingomonas sp.
4.2.2.3 recombinant protein Sphingomonas sp.
4.2.2.11 recombinant protein Sphingomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.3 additional information the organism is able to grow on alginate as the sole carbon source Sphingomonas sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 beta-D-mannuronyl linkage in alginate
-
 Sphingomonas sp. ? final degradation products are alginate monosaccharides ?
4.2.2.3 beta-D-mannuronyl linkage in alginate
-
 Sphingomonas sp. MJ-3 ? final degradation products are alginate monosaccharides ?
4.2.2.3 additional information substrate specificity of recombinant oligoalginate lyase, overview. Analysis of the final degradation products, overview Sphingomonas sp. ?
-
 ?
4.2.2.3 additional information substrate specificity of recombinant oligoalginate lyase, overview. Analysis of the final degradation products, overview Sphingomonas sp. MJ-3 ?
-
 ?
4.2.2.3 poly(beta-(1->4)-D-mannuronic acid) 190% of the activity with alginate Sphingomonas sp. ?
-
 ?
4.2.2.3 poly(beta-(1->4)-D-mannuronic acid) 190% of the activity with alginate Sphingomonas sp. MJ-3 ?
-
 ?
4.2.2.3 poly(beta-(1->4)-D-mannuronic acid/alpha-(1->4)-L-guluronic acid) alternating structure of alpha-L-guluronic acid and beta-D-mannuronic acid. 120% of the activity with alginate Sphingomonas sp. ?
-
 ?
4.2.2.3 poly(beta-(1->4)-D-mannuronic acid/alpha-(1->4)-L-guluronic acid) alternating structure of alpha-L-guluronic acid and beta-D-mannuronic acid. 120% of the activity with alginate Sphingomonas sp. MJ-3 ?
-
 ?
4.2.2.11 alpha-L-guluronosyl linkage in alginate
-
 Sphingomonas sp. ? final degradation products are alginate monosaccharides ?
4.2.2.11 alpha-L-guluronosyl linkage in alginate
-
 Sphingomonas sp. MJ-3 ? final degradation products are alginate monosaccharides ?
4.2.2.11 poly(alpha-(1->4)-L-guluronic acid) 90% of the activity with alginate Sphingomonas sp. ?
-
 ?
4.2.2.11 poly(beta-(1->4)-D-mannuronic acid/alpha-(1->4)-L-guluronic acid) alternating structure of alpha-L-guluronic acid and beta-D-mannuronic acid. 120% of the activity with alginate Sphingomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 ? x * 79600, recombinant His6-tagged enzyme, SDS-PAGE Sphingomonas sp.
4.2.2.3 ? x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE Sphingomonas sp.
4.2.2.11 ? x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE Sphingomonas sp.

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 MJ-3 alginate lyase
-
 Sphingomonas sp.
4.2.2.3 oligoalginate lyase
-
 Sphingomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 50
-
-
 Sphingomonas sp.
4.2.2.11 50
-
-
 Sphingomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.3 20 80 activity range Sphingomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 6.5
-
-
 Sphingomonas sp.
4.2.2.11 6.5
-
-
 Sphingomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.3 5 9 activity range Sphingomonas sp.