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Literature summary extracted from

  • Bacha, A.B.; Karray, A.; Bouchaala, E.; Gargouri, Y.; Ali, Y.B.
    Purification and biochemical characterization of pancreatic phospholipase A2 from the common stingray Dasyatis pastinaca (2011), Lipids Health Dis., 10, 32.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.1.4 food industry PLA2 stability in the presence of organic solvents, as well as in acidic and alkaline pH and at high temperature makes it a good candidate for its application in food industry Dasyatis pastinaca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.4 17
-
phosphatidylcholine at pH 8.5 and 40°C Dasyatis pastinaca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 14000
-
1 * 14000, SDS-PAGE Dasyatis pastinaca

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.4 2-propanol 97% and 93% residual activity after 1 h and 2 h, respectively, at 50% (v/v) Dasyatis pastinaca
3.1.1.4 Acetone 95% and 92% residual activity after 1 h and 2 h, respectively, at 50% (v/v) Dasyatis pastinaca
3.1.1.4 acetonitrile 105% activity at 50% (v/v) after 1 h and 2 h incubation Dasyatis pastinaca
3.1.1.4 Ethanol 116% and 100% residual activity after 1 h and 2 h, respectively, at 50% (v/v) Dasyatis pastinaca
3.1.1.4 Methanol 98% and 95% residual activity after 1 h and 2 h, respectively, at 50% (v/v) Dasyatis pastinaca

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Dasyatis pastinaca
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.4 no glycoprotein
-
Dasyatis pastinaca

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 ammonium sulfate precipitation, ethanol fractionation, Mono-S Sepharose column chromatography, Sephadex G-50 gel filtration, RP-HPLC C-8 column chromatography, and Mono-Q column chromatography Dasyatis pastinaca

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 pancreas
-
Dasyatis pastinaca
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 750
-
purified enzyme, in the presence of 4 mM sodium taurodeoxycholate and 8 mM CaCl2 using phosphatidylcholine as substrate, at pH 8.0 and 40°C Dasyatis pastinaca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 phosphatidylcholine + H2O
-
Dasyatis pastinaca 1-acylglycerophosphocholine + a carboxylate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.4 monomer 1 * 14000, SDS-PAGE Dasyatis pastinaca

Synonyms

EC Number Synonyms Comment Organism
3.1.1.4 pancreatic-type PLA2
-
Dasyatis pastinaca
3.1.1.4 PLA2
-
Dasyatis pastinaca
3.1.1.4 sPLA2-IB
-
Dasyatis pastinaca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.4 40
-
-
Dasyatis pastinaca

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.4 187
-
phosphatidylcholine at pH 8.5 and 40°C Dasyatis pastinaca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 8.5
-
-
Dasyatis pastinaca