EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.1.4 | food industry | PLA2 stability in the presence of organic solvents, as well as in acidic and alkaline pH and at high temperature makes it a good candidate for its application in food industry | Dasyatis pastinaca |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.4 | 17 | - |
phosphatidylcholine | at pH 8.5 and 40°C | Dasyatis pastinaca |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 14000 | - |
1 * 14000, SDS-PAGE | Dasyatis pastinaca |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.1.1.4 | 2-propanol | 97% and 93% residual activity after 1 h and 2 h, respectively, at 50% (v/v) | Dasyatis pastinaca |
3.1.1.4 | Acetone | 95% and 92% residual activity after 1 h and 2 h, respectively, at 50% (v/v) | Dasyatis pastinaca |
3.1.1.4 | acetonitrile | 105% activity at 50% (v/v) after 1 h and 2 h incubation | Dasyatis pastinaca |
3.1.1.4 | Ethanol | 116% and 100% residual activity after 1 h and 2 h, respectively, at 50% (v/v) | Dasyatis pastinaca |
3.1.1.4 | Methanol | 98% and 95% residual activity after 1 h and 2 h, respectively, at 50% (v/v) | Dasyatis pastinaca |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.4 | Dasyatis pastinaca | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.1.4 | no glycoprotein | - |
Dasyatis pastinaca |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.4 | ammonium sulfate precipitation, ethanol fractionation, Mono-S Sepharose column chromatography, Sephadex G-50 gel filtration, RP-HPLC C-8 column chromatography, and Mono-Q column chromatography | Dasyatis pastinaca |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.4 | pancreas | - |
Dasyatis pastinaca | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 750 | - |
purified enzyme, in the presence of 4 mM sodium taurodeoxycholate and 8 mM CaCl2 using phosphatidylcholine as substrate, at pH 8.0 and 40°C | Dasyatis pastinaca |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.4 | phosphatidylcholine + H2O | - |
Dasyatis pastinaca | 1-acylglycerophosphocholine + a carboxylate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.4 | monomer | 1 * 14000, SDS-PAGE | Dasyatis pastinaca |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.4 | pancreatic-type PLA2 | - |
Dasyatis pastinaca |
3.1.1.4 | PLA2 | - |
Dasyatis pastinaca |
3.1.1.4 | sPLA2-IB | - |
Dasyatis pastinaca |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 40 | - |
- |
Dasyatis pastinaca |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.4 | 187 | - |
phosphatidylcholine | at pH 8.5 and 40°C | Dasyatis pastinaca |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 8.5 | - |
- |
Dasyatis pastinaca |