EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.1.15 | expressed in Escherichia coli ER2566 cells | Serratia proteamaculans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.15 | Cu2+ | - |
Serratia proteamaculans | |
5.3.1.15 | EDTA | - |
Serratia proteamaculans | |
5.3.1.15 | Fe2+ | - |
Serratia proteamaculans | |
5.3.1.15 | Ni2+ | - |
Serratia proteamaculans | |
5.3.1.15 | Zn2+ | - |
Serratia proteamaculans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.15 | 3.83 | - |
D-xylulose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans | |
5.3.1.15 | 13.3 | - |
D-Lyxose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans | |
5.3.1.15 | 19.4 | - |
D-fructose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans | |
5.3.1.15 | 32.2 | - |
D-mannose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.15 | Ca2+ | the addition of Ca2+ ion to EDTA-treated enzyme enhances the activity of D-lyxose isomerization | Serratia proteamaculans | |
5.3.1.15 | Mn2+ | maximum activity (190%) in the presence of 1 mM Mn2+ | Serratia proteamaculans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.3.1.15 | 26756 | - |
2 * 26756, calculated from amino acid sequence | Serratia proteamaculans |
5.3.1.15 | 27000 | - |
2 * 27000, SDS-PAGE | Serratia proteamaculans |
5.3.1.15 | 54000 | - |
gel filtration | Serratia proteamaculans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.15 | Serratia proteamaculans | D5MTT1 | - |
- |
5.3.1.15 | Serratia proteamaculans KCTC 2936 | D5MTT1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.1.15 | His-Trap column chromatography | Serratia proteamaculans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.3.1.15 | 0.02 | - |
using L-sorbose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
5.3.1.15 | 0.14 | - |
using L-ribose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
5.3.1.15 | 0.19 | - |
using D-talose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
5.3.1.15 | 0.28 | - |
using D-tagatose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
5.3.1.15 | 0.4 | - |
using L-ribulose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
5.3.1.15 | 1.05 | - |
using L-gulose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
5.3.1.15 | 1.41 | - |
using D-fructose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
5.3.1.15 | 5.42 | - |
using D-mannose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
5.3.1.15 | 10 | - |
using D-lyxose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
5.3.1.15 | 26.2 | - |
using D-xylulose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.15 | D-fructose | - |
Serratia proteamaculans | D-mannose | - |
r | |
5.3.1.15 | D-Lyxose | highest activity | Serratia proteamaculans | D-Xylulose | - |
r | |
5.3.1.15 | D-Lyxose | highest activity | Serratia proteamaculans KCTC 2936 | D-Xylulose | - |
r | |
5.3.1.15 | D-Mannose | - |
Serratia proteamaculans | D-Fructose | - |
r | |
5.3.1.15 | D-Mannose | - |
Serratia proteamaculans KCTC 2936 | D-Fructose | - |
r | |
5.3.1.15 | D-talose | - |
Serratia proteamaculans | D-tagatose | - |
r | |
5.3.1.15 | D-talose | - |
Serratia proteamaculans KCTC 2936 | D-tagatose | - |
r | |
5.3.1.15 | D-xylulose | - |
Serratia proteamaculans | D-lyxose | - |
r | |
5.3.1.15 | L-gulose | - |
Serratia proteamaculans | L-sorbose | - |
r | |
5.3.1.15 | L-ribose | - |
Serratia proteamaculans | L-ribulose | - |
r | |
5.3.1.15 | L-ribose | - |
Serratia proteamaculans KCTC 2936 | L-ribulose | - |
r | |
5.3.1.15 | additional information | no activity with D-mannose 6-phosphate, D-glucose 6-phosphate, and D-ribose 6-phosphate | Serratia proteamaculans | ? | - |
? | |
5.3.1.15 | additional information | no activity with D-mannose 6-phosphate, D-glucose 6-phosphate, and D-ribose 6-phosphate | Serratia proteamaculans KCTC 2936 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.1.15 | homodimer | 2 * 27000, SDS-PAGE | Serratia proteamaculans |
5.3.1.15 | homodimer | 2 * 26756, calculated from amino acid sequence | Serratia proteamaculans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.1.15 | D-Lyxose isomerase | - |
Serratia proteamaculans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.15 | 40 | - |
- |
Serratia proteamaculans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.15 | 30 | 50 | the enzyme shows half-lives of 84 h, 17 h, 2.6 h, 0.3 h, and 0.09 h at 30°C, 35°C, 40°C, 45°C, and 50°C, respectively | Serratia proteamaculans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.15 | 4297 | - |
D-fructose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans | |
5.3.1.15 | 16170 | - |
D-mannose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans | |
5.3.1.15 | 30810 | - |
D-Lyxose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans | |
5.3.1.15 | 31620 | - |
D-xylulose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.15 | 7.5 | - |
- |
Serratia proteamaculans |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.15 | 222 | - |
D-fructose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans | |
5.3.1.15 | 502 | - |
D-mannose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans | |
5.3.1.15 | 2333 | - |
D-Lyxose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans | |
5.3.1.15 | 9560 | - |
D-xylulose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |