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Literature summary extracted from
- Graminan breakdown by fructan exohydrolase induced in winter wheat inoculated with snow mold (2012), J. Plant Physiol., 169, 294-302.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.153 | gene Wfh-sm3, recombinant expression in Pichia pastoris | Triticum aestivum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.153 | additional information | Triticum aestivum | isozyme Wfh-sm3m hydrolyzes beta-(2,6)-linkages of fructans (6-kestotriose) more efficiently than beta-(2,1)-linkages of fructans (1-kestotriose and 1,1-kestotetraose). On the other hand, Wfh-sm3m cannot hydrolyze long-chain fructans such as inulin and bacterial levan | ? | - |
? |  |
| 3.2.1.153 | wheat graminan + H2O | Triticum aestivum | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.80 | Triticum aestivum | - |
- |
- |
| 3.2.1.153 | Triticum aestivum | - |
cultivars PI 173438 (snow mold-resistant) and Valuevskaya (snow mold-susceptible), gene Wfh-sm3 | - |
| 3.2.1.154 | Triticum aestivum | - |
cultivars PI 173438 (snow mold-resistant) and Valuevskaya (snow mold-susceptible) | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.80 | leaf | - |
Triticum aestivum | - |
| 3.2.1.153 | leaf | - |
Triticum aestivum | - |
| 3.2.1.154 | leaf | - |
Triticum aestivum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.80 | 1,1-kestotetraose + H2O | gene product Wfh-sm1 shows 8% activity with 1,1-kestotetraose compared to sucrose | Triticum aestivum | ? | - |
? | |
| 3.2.1.80 | 1-kestotriose + H2O | gene product Wfh-sm1 shows 40% activity with 1-kestotriose compared to sucrose | Triticum aestivum | ? | - |
? | |
| 3.2.1.80 | 6-kestotriose + H2O | gene product Wfh-sm1 shows 38% activity with 6-kestotriose compared to sucrose | Triticum aestivum | ? | - |
? | |
| 3.2.1.80 | inulin + H2O | gene product Wfh-sm1 shows less than 1% activity with inulin compared to sucrose | Triticum aestivum | ? | - |
? | |
| 3.2.1.80 | levan + H2O | gene product Wfh-sm1 shows less than 1% activity with levan compared to sucrose | Triticum aestivum | ? | - |
? | |
| 3.2.1.80 | sucrose + H2O | 100% activity | Triticum aestivum | beta-D-fructose + alpha-D-glucose | - |
? | |
| 3.2.1.153 | 1,1-kestotetraose + H2O | - |
Triticum aestivum | ? | - |
? | |
| 3.2.1.153 | 1-kestotriose + H2O | - |
Triticum aestivum | ? | - |
? | |
| 3.2.1.153 | 6-kestotriose + H2O | - |
Triticum aestivum | ? | - |
? | |
| 3.2.1.153 | additional information | isozyme Wfh-sm3m hydrolyzes beta-(2,6)-linkages of fructans (6-kestotriose) more efficiently than beta-(2,1)-linkages of fructans (1-kestotriose and 1,1-kestotetraose). On the other hand, Wfh-sm3m cannot hydrolyze long-chain fructans such as inulin and bacterial levan | Triticum aestivum | ? | - |
? | |
| 3.2.1.153 | additional information | wheat graminan degradation by Wfh-sm3 recombinant protein Wfh-sm3m. The recombinant protein Wfh-sm3m preferentially degrades 6-kestotriose and possesses minor hydrolase activity to 1-kestotriose and 1,1-kestotetraose, poor activity with sucrose, and no activity with inulin and levan | Triticum aestivum | ? | - |
? | |
| 3.2.1.153 | wheat graminan + H2O | - |
Triticum aestivum | ? | - |
? | |
| 3.2.1.154 | 1,1-kestotetraose + H2O | minor hydrolase activity (16% activity compared to 6-kestotriose) | Triticum aestivum | ? | - |
? | |
| 3.2.1.154 | 1-kestotriose + H2O | minor hydrolase activity (8% activity compared to 6-kestotriose) | Triticum aestivum | ? | - |
? | |
| 3.2.1.154 | 6-kestotriose + H2O | preferred substrate (100% activity) | Triticum aestivum | ? | - |
? | |
| 3.2.1.154 | graminan + H2O | - |
Triticum aestivum | ? | - |
? | |
| 3.2.1.154 | additional information | less than 1% activity towards inulin and levan compared to 6-kestotriose (poor invertase activity) | Triticum aestivum | ? | - |
? | |
| 3.2.1.154 | sucrose + H2O | 2% activity compared to 6-kestotriose | Triticum aestivum | D-fructose + D-glucose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.80 | fructan exohydrolase | - |
Triticum aestivum |
| 3.2.1.80 | Wfh-sm1 | - |
Triticum aestivum |
| 3.2.1.153 | 1-FEH | - |
Triticum aestivum |
| 3.2.1.153 | FEH | - |
Triticum aestivum |
| 3.2.1.153 | fructan exohydrolase | - |
Triticum aestivum |
| 3.2.1.153 | Wfh-sm3m | - |
Triticum aestivum |
| 3.2.1.154 | 6-FEH | - |
Triticum aestivum |
| 3.2.1.154 | 6-fructan exohydrolase | - |
Triticum aestivum |
| 3.2.1.154 | Wfh-sm3 | the recombinant protein is a 6-fructan exohydrolase with minor 1-fructan exohydrolase activity | Triticum aestivum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.153 | 5 | 35 | assay at | Triticum aestivum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.153 | 5 | - |
assay at | Triticum aestivum |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.2.1.154 | Triticum aestivum | Wfh-sm3 transcripts increase in wheat leaf tissues inoculated with snow mold and incubated under snow cover | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.1.153 | metabolism | snow molds consume carbon sources contained in the inoculated wheat tissues but cannot effectively use wheat polysaccharide including fructans compared with mono- and disaccharides. Therefore, in snow mold inoculated wheat tissues, fructans are rapidly degraded, mainly by wheat enzymes, FEHs, to maintain necessary levels of mono- and disaccharides for metabolic demands under snow cover | Triticum aestivum |
| 3.2.1.154 | physiological function | the enzyme is associated with degradation of fructans in wheat leaf tissues during inoculation and incubation under snow cover | Triticum aestivum |
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Release 2023.1 (January 2023)
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