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Literature summary extracted from

  • Moreno-Perez, A.J.; Martinez-Force, E.; Garces, R.; Salas, J.J.
    Phospholipase Dalpha from sunflower (Helianthus annuus): cloning and functional characterization (2010), J. Plant Physiol., 167, 503-511.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.4 gene HaPLDalpha, cloning from leaf DNA, DNA and amino acid sequence determination and analysis, expression of His-tagged PLDalpha in Escherichia coli Helianthus annuus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Ca2+ recombinant PLDalpha, dependent on Helianthus annuus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Helianthus annuus
-
 gene HaPLDalpha
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4 recombinant His-tagged PLDalpha from Escherichia coli by nickel affinity chromatography to homogeneity Helianthus annuus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.4 cotyledon PLDalpha Helianthus annuus
-
3.1.4.4 leaf high expression level of PLDalpha Helianthus annuus
-
3.1.4.4 root PLDalpha Helianthus annuus
-
3.1.4.4 seed PLDalpha Helianthus annuus
-
3.1.4.4 stem high expression level of PLDalpha Helianthus annuus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 phosphatidylcholine + H2O
-
 Helianthus annuus choline + phosphatidate
-
 ?
3.1.4.4 phosphatidylethanolamine + H2O
-
 Helianthus annuus ethanolamine + phosphatidate
-
 ?
3.1.4.4 phosphatidylglycerol + H2O
-
 Helianthus annuus glycerol + phosphatidate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.4 D type phospholipase
-
 Helianthus annuus
3.1.4.4 phospholipase Dalpha
-
 Helianthus annuus
3.1.4.4 PLD
-
 Helianthus annuus
3.1.4.4 PLDalpha
-
 Helianthus annuus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 6.5
-
 recombinant PLDalpha Helianthus annuus

General Information

EC Number General Information Comment Organism
3.1.4.4 physiological function D type phospholipases are enzymes that hydrolyze the head group of phospholipids to produce phosphatidic acid Helianthus annuus