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Literature summary extracted from

  • Whalen, K.L.; Tussey, K.B.; Blanke, S.R.; Spies, M.A.
    Nature of allosteric inhibition in glutamate racemase: discovery and characterization of a cryptic inhibitory pocket using atomistic MD simulations and pKa calculations (2011), J. Phys. Chem. B, 115, 3416-3424.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.3 expressed in Escherichia coli BL21(DE3) cells Bacillus anthracis

Protein Variants

EC Number Protein Variants Comment Organism
5.1.1.3 K106A the isozyme RacE2 mutant is insensitive to dipicolinic acid Bacillus anthracis
5.1.1.3 S207A the isozyme RacE2 mutant is insensitive to dipicolinic acid Bacillus anthracis

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.3 dipicolinic acid allosteric inhibitor of both Bacillus anthracis glutamate racemase isozymes which exhibits low micromolar inhibition with clear noncompetitive behavior Bacillus anthracis

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.3 Bacillus anthracis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.3 ammonium sulfate precipitation, Co2+-affinity column chromatography, and anion-exchange chromatography Bacillus anthracis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.3 D-glutamate
-
 Bacillus anthracis L-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.1.1.3 RacE1 isozyme Bacillus anthracis
5.1.1.3 RacE2 isozyme Bacillus anthracis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.3 8
-
-
 Bacillus anthracis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.1.1.3 0.075
-
 dipicolinic acid isozyme RacE1, pH and temperature not specified in the publication Bacillus anthracis
5.1.1.3 0.092
-
 dipicolinic acid isozyme RacE2, pH and temperature not specified in the publication Bacillus anthracis
5.1.1.3 1.236
-
 dipicolinic acid RacE2 mutant enzyme S207A, pH and temperature not specified in the publication Bacillus anthracis
5.1.1.3 2.342
-
 dipicolinic acid RacE2 mutant enzyme K106A, pH and temperature not specified in the publication Bacillus anthracis