EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.53 | adenosine 3',5'-cyclic phosphate + H2O | Homo sapiens | determination of reaction rate and mechanism using computational modeling, quantum mechanical/molecular mechanical-free energy perturbation, QM/MM-FE, and QM/MM-Poisson-Boltzmann surface area, PBSA, calculations. The onQM/MMreaction-coordinate calculations including the protein environment of any PDE-catalyzed reaction system identifies a unique catalytic reaction mechanism, overview | adenosine 5'-phosphate | - |
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EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.53 | Homo sapiens | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.4.53 | adenosine 3',5'-cyclic phosphate + H2O = AMP | catalytic reaction mechanism, overview | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.53 | adenosine 3',5'-cyclic phosphate + H2O | - |
Homo sapiens | adenosine 5'-phosphate | - |
? | |
3.1.4.53 | adenosine 3',5'-cyclic phosphate + H2O | determination of reaction rate and mechanism using computational modeling, quantum mechanical/molecular mechanical-free energy perturbation, QM/MM-FE, and QM/MM-Poisson-Boltzmann surface area, PBSA, calculations. The onQM/MMreaction-coordinate calculations including the protein environment of any PDE-catalyzed reaction system identifies a unique catalytic reaction mechanism, overview | Homo sapiens | adenosine 5'-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.53 | PDE4 | - |
Homo sapiens |
3.1.4.53 | phosphodiesterase-4 | - |
Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.4.53 | physiological function | the PDE4-catalyzed hydrolysis of cAMP consists of two reaction stages: cAMP hydrolysis and bridging hydroxide ion regeneration. The stage 1 includes the binding of cAMP in the active site, nucleophilic attack of the bridging hydroxide ion on the phosphorus atom of cAMP, cleavage of O3'-P phosphoesteric bond of cAMP, protonation of the departing O3' atom, and dissociation of hydrolysis product, AMP. The stage 2 includes the binding of solvent water molecules with the metal ions in the active site and regeneration of the bridging hydroxide ion. The dissociation of the hydrolysis product is found to be rate-determining for the enzymatic reaction process | Homo sapiens |