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Literature summary extracted from

  • Sang, S.L.; Li, G.; Hu, X.P.; Liu, Y.H.
    Molecular cloning, overexpression and characterization of a novel feruloyl esterase from a soil metagenomic library (2011), J. Mol. Microbiol. Biotechnol., 20, 196-203.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 gene estF27, cloning from a soil metagenomic library, DNA and amino acid sequence determination and analysis, phylogenetic analysis of EstF27 uncultured bacterium

General Stability

EC Number General Stability Organism
3.1.1.73 the type A feruloyl esterase EstF27 retains more than 50% of its activity after 96 or 120 h incubation in the presence of 3 M KCl or 5 M NaCl uncultured bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.73 Al3+ 8% inhibition at 1 mM, 25% inhibition at 10 mM uncultured bacterium
3.1.1.73 Co2+ no activation at 1 mM, 11% at 5 mM, but 30% inhibition at 10 mM uncultured bacterium
3.1.1.73 Cu2+ 70% inhibition at 1 mM, complete inhibition at 5-10 mM uncultured bacterium
3.1.1.73 Fe3+ 23% activation at 1 mM, 13% at 5 mM, 78% inhibition at 10 mM uncultured bacterium
3.1.1.73 Mn2+ 10% inhibition at 1 mM, 26% inhibition at 10 mM uncultured bacterium
3.1.1.73 Zn2+ 24% inhibition at 1 mM, 61% inhibition at 10 mM uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.158
-
4-nitrophenyl butyrate pH 6.8, 40°C uncultured bacterium
3.1.1.73 0.261
-
4-nitrophenyl acetate pH 6.8, 40°C uncultured bacterium
3.1.1.73 0.5
-
4-nitrophenyl ferulate pH 6.8, 40°C uncultured bacterium
3.1.1.73 0.56
-
methyl sinapate pH 6.8, 40°C uncultured bacterium
3.1.1.73 0.83
-
methyl ferulate pH 6.8, 40°C uncultured bacterium
3.1.1.73 1.25
-
methyl 4-coumarate pH 6.8, 40°C uncultured bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.73 Co2+ no activation at 1 mM, 11% at 5 mM, but 30% inhibition at 10 mM uncultured bacterium
3.1.1.73 Fe3+ 23% activation at 1 mM, 13% at 5 mM, 78% inhibition at 10 mM uncultured bacterium
3.1.1.73 Mg2+ 22% activation at 1 mM, 6% at 10 mM uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 uncultured bacterium E7DJY5 gene estF27
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 0.84
-
recombinant enzyme, substrate 4-nitrophenyl decanoate, pH 6.8, 40°C uncultured bacterium
3.1.1.73 2.5
-
recombinant enzyme, substrate methyl 4-coumarate, pH 6.8, 40°C uncultured bacterium
3.1.1.73 7.3
-
recombinant enzyme, substrate methyl ferulate, pH 6.8, 40°C uncultured bacterium
3.1.1.73 9.1
-
recombinant enzyme, substrate 4-nitrophenyl octanoate, pH 6.8, 40°C uncultured bacterium
3.1.1.73 10.2
-
recombinant enzyme, substrate methyl sinapate, pH 6.8, 40°C uncultured bacterium
3.1.1.73 21.7
-
recombinant enzyme, substrate 4-nitrophenyl ferulate, pH 6.8, 40°C uncultured bacterium
3.1.1.73 136.5
-
recombinant enzyme, substrate 4-nitrophenyl acetate, pH 6.8, 40°C uncultured bacterium
3.1.1.73 219.1
-
recombinant enzyme, substrate 4-nitrophenyl butyrate, pH 6.8, 40°C uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 4-nitrophenyl acetate + H2O
-
uncultured bacterium 4-nitrophenol + acetate
-
?
3.1.1.73 4-nitrophenyl butyrate + H2O
-
uncultured bacterium 4-nitrophenol + butyrate
-
?
3.1.1.73 4-nitrophenyl decanoate + H2O
-
uncultured bacterium 4-nitrophenol + decanoate
-
?
3.1.1.73 4-nitrophenyl ferulate + H2O
-
uncultured bacterium 4-nitrophenol + ferulate
-
?
3.1.1.73 4-nitrophenyl octanoate + H2O
-
uncultured bacterium 4-nitrophenol + octanoate
-
?
3.1.1.73 methyl 4-coumarate + H2O
-
uncultured bacterium 4-coumaric acid + methanol
-
?
3.1.1.73 methyl ferulate + H2O
-
uncultured bacterium ferulic acid + methanol
-
?
3.1.1.73 methyl sinapate + H2O
-
uncultured bacterium sinapic acid + methanol
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 EstF27
-
uncultured bacterium
3.1.1.73 FAE
-
uncultured bacterium
3.1.1.73 type A feruloyl esterase
-
uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 40
-
-
uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 40
-
80% of maximal activity after 192 h, pH 6.8 uncultured bacterium
3.1.1.73 45
-
half-life is 72 h, inactivation after 96 h, pH 6.8 uncultured bacterium
3.1.1.73 50
-
half-life is 7 h, pH 6.8 uncultured bacterium
3.1.1.73 55
-
half-life is 0.08 h, inactivation after 0.5 h, pH 6.8 uncultured bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 7.5
-
methyl 4-coumarate pH 6.8, 40°C uncultured bacterium
3.1.1.73 16.2
-
methyl sinapate pH 6.8, 40°C uncultured bacterium
3.1.1.73 19.1
-
methyl ferulate pH 6.8, 40°C uncultured bacterium
3.1.1.73 19.7
-
4-nitrophenyl ferulate pH 6.8, 40°C uncultured bacterium
3.1.1.73 63.2
-
4-nitrophenyl acetate pH 6.8, 40°C uncultured bacterium
3.1.1.73 203.2
-
4-nitrophenyl butyrate pH 6.8, 40°C uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 6.8
-
-
uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 4 10 activity range, profile, overview uncultured bacterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.73 5 10 the type A feruloyl esterase EstF27 is stable in a broad pH range of 5.0-10.0 over 24 h, most stable at pH 6.5-8.0 uncultured bacterium

General Information

EC Number General Information Comment Organism
3.1.1.73 additional information EstF27 contains one conserved active site of pentapeptide motif G-X-S-XG, amino acid positions from 149 to 153, and a putative catalytic triad comprising His73, Asp123 and Ser151 in the G-X-S-X-G uncultured bacterium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.73 23.1
-
methyl ferulate pH 6.8, 40°C uncultured bacterium
3.1.1.73 28.9
-
methyl sinapate pH 6.8, 40°C uncultured bacterium
3.1.1.73 39.4
-
4-nitrophenyl ferulate pH 6.8, 40°C uncultured bacterium
3.1.1.73 242
-
4-nitrophenyl acetate pH 6.8, 40°C uncultured bacterium
3.1.1.73 681.5
-
methyl 4-coumarate pH 6.8, 40°C uncultured bacterium
3.1.1.73 1286
-
4-nitrophenyl butyrate pH 6.8, 40°C uncultured bacterium