EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.2 | expressed in Escherichia coli as a His-tagged fusion protein | Francisella tularensis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.2 | 0.15 | - |
Thiamine monophosphate | pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 13.3 micromol/min/mg | Francisella tularensis | |
3.1.3.2 | 0.19 | - |
thiamine diphosphate | pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 1.38 micromol/min/mg | Francisella tularensis | |
3.1.3.2 | 0.47 | - |
phosphocholine | pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 89.58 micromol/min/mg | Francisella tularensis | |
3.1.3.2 | 3.11 | - |
4-nitrophenyl phosphate | pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 11.84 micromol/min/mg | Francisella tularensis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.2 | Co2+ | improves activity | Francisella tularensis | |
3.1.3.2 | Fe2+ | improves activity | Francisella tularensis | |
3.1.3.2 | Mg2+ | improves activity | Francisella tularensis | |
3.1.3.2 | Mn2+ | improves activity | Francisella tularensis | |
3.1.3.2 | Zn2+ | improves activity | Francisella tularensis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.2 | Francisella tularensis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.2 | using Ni-NTA chromatography | Francisella tularensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.2 | 4-nitrophenyl phosphate + H2O | - |
Francisella tularensis | 4-nitrophenol + phosphate | - |
? | |
3.1.3.2 | phosphocholine + H2O | - |
Francisella tularensis | choline + phosphate | - |
? | |
3.1.3.2 | thiamine diphosphate + 2 H2O | - |
Francisella tularensis | thiamine + 2 phosphate | - |
? | |
3.1.3.2 | thiamine monophosphate + H2O | - |
Francisella tularensis | thiamine + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.2 | AcpB | - |
Francisella tularensis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.2 | 0.48 | - |
thiamine diphosphate | pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 1.38 micromol/min/mg | Francisella tularensis | |
3.1.3.2 | 4.48 | - |
4-nitrophenyl phosphate | pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 11.84 micromol/min/mg | Francisella tularensis | |
3.1.3.2 | 4.85 | - |
Thiamine monophosphate | pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 13.3 micromol/min/mg | Francisella tularensis | |
3.1.3.2 | 33.24 | - |
phosphocholine | pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 89.58 micromol/min/mg | Francisella tularensis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 5.2 | 5.6 | - |
Francisella tularensis |