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Literature summary extracted from

  • Bao, L.; Huang, Q.; Chang, L.; Zhou, J.; Lu, H.
    Screening and characterization of a cellulase with endocellulase and exocellulase activity from yak rumen metagenome (2011), J. Mol. Catal. B, 73, 104-110.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression of His-tagged Rucel5B in Escherichia coli strain BL21(DE3) uncultured rumen bacterium
3.2.1.74 expressed in Escherichia coli BL21(DE3) cells uncultured bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Ca2+
-
 uncultured rumen bacterium
3.2.1.4 Cu2+
-
 uncultured rumen bacterium
3.2.1.74 Ca2+ the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Ca2+ uncultured bacterium
3.2.1.74 Cu2+
-
 uncultured bacterium
3.2.1.74 Mg2+ the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Mg2+ uncultured bacterium
3.2.1.74 Ni2+ the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Ni2+ uncultured bacterium
3.2.1.74 Zn2+ the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Zn2+ uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 additional information kinetics of Rucel5B with different substrates, overview uncultured rumen bacterium
3.2.1.74 1.74
-
 4-nitrophenyl beta-D-cellobioside at 65°C in pH 5.5 citrate buffer uncultured bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Co2+ activates uncultured rumen bacterium
3.2.1.4 Mg2+ activates uncultured rumen bacterium
3.2.1.4 additional information no effect by Zn2+ on Rucel5B activity uncultured rumen bacterium
3.2.1.4 Ni2+ activates uncultured rumen bacterium
3.2.1.74 additional information the addition of 5 mM Zn2+, Co2+ and Cu2+ has no statistical difference on enzyme activity uncultured bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 38400
-
 x * 40000, recombinant His-tagged Rucel5B, SDS-PAGE, x * 38400, about, Rucel5B, sequence calculation uncultured rumen bacterium
3.2.1.4 40000
-
 x * 40000, recombinant His-tagged Rucel5B, SDS-PAGE, x * 38400, about, Rucel5B, sequence calculation uncultured rumen bacterium
3.2.1.74 38400
-
 x * 38400, calculated from amino acid sequence uncultured bacterium
3.2.1.74 40000
-
 x * 40000, SDS-PAGE uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 uncultured rumen bacterium G3BJX5 from rumen of Bos mutus, Rucel5B
-
3.2.1.74 uncultured bacterium
-
 from yak rumen
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 recombinant His-tagged Rucel5B from Escherichia coli strain BL21(DE3) by nickel affinity chromatography uncultured rumen bacterium
3.2.1.74 Ni-NTA resincolumn chromatography uncultured bacterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 220
-
 Rucel5B, pH 6.5, 60°C, substrate carboxymethyl cellulose uncultured rumen bacterium
3.2.1.74 0.04
-
 using filter paper as substrate, at 60°C in pH 6.5 citrate buffer uncultured bacterium
3.2.1.74 0.2
-
 using avicel as substrate, at 60°C in pH 6.5 citrate buffer uncultured bacterium
3.2.1.74 23.5
-
 using phosphate acid swollen cellulose as substrate, at 60°C in pH 6.5 citrate buffer uncultured bacterium
3.2.1.74 52.9
-
 using 4-nitrophenyl beta-D-cellobioside as substrate, at 65°C in pH 5.5 citrate buffer uncultured bacterium
3.2.1.74 220
-
 using carboxymethylcellulose as substrate, at 65°C in pH 5.5 citrate buffer uncultured bacterium
3.2.1.74 693
-
 using lichenan as substrate, at 60°C in pH 6.5 citrate buffer uncultured bacterium
3.2.1.74 717
-
 using barley glucan as substrate, at 60°C in pH 6.5 citrate buffer uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
 uncultured rumen bacterium ?
-
 ?
3.2.1.4 additional information Rucel5B is active towards carboxymethyl cellulose, barley glucan, lichenan, pNPC, PASC, avicel and Whatman NO.1 filter paper, but not active towards laminarin, beta-1,6-glucan and birchwood xylan uncultured rumen bacterium ?
-
 ?
3.2.1.74 4-nitrophenyl beta-D-cellobioside + H2O exo-beta-1,4-glucanase activity, 24.1% activity compared to carboxymethylcellulose uncultured bacterium 4-nitrophenol + beta-D-cellobiose
-
 ?
3.2.1.74 avicel + H2O 0.09% activity compared to carboxymethylcellulose uncultured bacterium cellobiose + ?
-
 ?
3.2.1.74 barley glucan + H2O 326% activity compared to carboxymethylcellulose uncultured bacterium ?
-
 ?
3.2.1.74 carboxymethylcellulose + H2O endo-beta-1,4-glucanase activity, 100% activity uncultured bacterium ?
-
 ?
3.2.1.74 cellotetraose + H2O
-
 uncultured bacterium cellobiose + cellotriose + D-glucose cellobiose is the dominant product, while cellotriose and glucose are minor products ?
3.2.1.74 filter paper + H2O 0.02% activity compared to carboxymethylcellulose uncultured bacterium cellobiose + ?
-
 ?
3.2.1.74 lichenan + H2O 315% activity compared to carboxymethylcellulose uncultured bacterium ?
-
 ?
3.2.1.74 additional information no activity with laminarin, glucan, birchwood xylan, and 4-nitrophenyl beta-D-glucopyranside uncultured bacterium ?
-
 ?
3.2.1.74 additional information the cellulase Rucel5B possesses endo-beta-1,4-glucanase activity and exo-beta-1,4-glucanase activity uncultured bacterium ?
-
 ?
3.2.1.74 phosphate acid swollen cellulose + H2O 10.7% activity compared to carboxymethylcellulose uncultured bacterium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 40000, recombinant His-tagged Rucel5B, SDS-PAGE, x * 38400, about, Rucel5B, sequence calculation uncultured rumen bacterium
3.2.1.74 ? x * 40000, SDS-PAGE uncultured bacterium
3.2.1.74 ? x * 38400, calculated from amino acid sequence uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 beta-1,4-endoglucanase
-
 uncultured rumen bacterium
3.2.1.4 Rucel5B
-
 uncultured rumen bacterium
3.2.1.74 endo/exocellulase
-
 uncultured bacterium
3.2.1.74 exo-bets-1,4-glucanase
-
 uncultured bacterium
3.2.1.74 Rucel5B
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 60
-
-
 uncultured rumen bacterium
3.2.1.74 60
-
 using carboxymethylcellulose as substrate uncultured bacterium
3.2.1.74 65
-
 using 4-nitrophenyl beta-D-cellobioside as substrate uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 30 80 activity range of Rucel5B, profile, overview uncultured rumen bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.74 60
-
 the melting temperature of the enzyme is at 60°C uncultured bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.4 163
-
 carboxymethyl cellulose Rucel5B, pH 6.5, 60°C uncultured rumen bacterium
3.2.1.74 69.6
-
 4-nitrophenyl beta-D-cellobioside at 65°C in pH 5.5 citrate buffer uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6.5
-
-
 uncultured rumen bacterium
3.2.1.74 5.5 6.5 using 4-nitrophenyl beta-D-cellobioside as substrate uncultured bacterium
3.2.1.74 6.5
-
 using carboxymethylcellulose as substrate uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 4 8 activity range of Rucel5B, profile, overview uncultured rumen bacterium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.74 40.1
-
 4-nitrophenyl beta-D-cellobioside at 65°C in pH 5.5 citrate buffer uncultured bacterium