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Literature summary extracted from

  • Giorgi, L.; Plateau, P.; O'Mahony, G.; Aubard, C.; Fromant, M.; Thureau, A.; Grotli, M.; Blanquet, S.; Bontems, F.
    NMR-based substrate analog docking to Escherichia coli peptidyl-tRNA hydrolase (2011), J. Mol. Biol., 412, 619-633.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.29 expressed in Escherichia coli strain K37DELTArecAlambdaDE3 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.29 H20A the mutant is unable to hydrolyze 2'(3')-O-L-(N,N-diacetyl-lysinyl)adenosine Escherichia coli
3.1.1.29 N10A the mutant shows strongly reduced activity with diacetyl-lysyl-tRNALys and L-Lys-tRNALys compared to the wild type enzyme Escherichia coli
3.1.1.29 N10D the mutant shows strongly reduced activity with diacetyl-lysyl-tRNALys and L-Lys-tRNALys compared to the wild type enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.29 3'-L-(N,N-diacetyl-lysinyl)amino-3'-deoxyadenosine
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.29 Escherichia coli P0A7D1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.29 ammonium sulfate precipitation Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.29 2'(3')-O-L-(N,N-diacetyl-lysinyl)adenosine + H2O minimalist substrate Escherichia coli ?
-
 ?
3.1.1.29 diacetyl-lysyl-tRNALys + H2O diacetyl-lysyl-tRNALys is hydrolyzed by the wild type enzyme 360fold more efficiently than Lys-tRNALys Escherichia coli diacetyl-lysine + tRNALys
-
 ?
3.1.1.29 L-Lys-tRNALys + H2O
-
 Escherichia coli L-lysine + tRNALys
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.29 peptidyl-tRNA hydrolase
-
 Escherichia coli
3.1.1.29 PTH
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.29 12
-
 3'-L-(N,N-diacetyl-lysinyl)amino-3'-deoxyadenosine at pH 6.0, with 50 mM sodium acetate and 200 mM NaCl, at 28°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.29 0.51
-
 2'(3')-O-L-(N,N-diacetyl-lysinyl)adenosine wild type enzyme, 28°C, 20 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 0.1 mM EDTA, 0.1 mM dithiothreitol Escherichia coli