EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.3.3 | His-tagged version expressed in Escherichia coli BL21 | Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.1.3.3 | sitting drop diffusion method, PEG 3350 | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.3.3 | E192A | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
4.1.3.3 | E192C | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
4.1.3.3 | E192D | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
4.1.3.3 | E192F | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
4.1.3.3 | E192G | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
4.1.3.3 | E192H | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
4.1.3.3 | E192I | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
4.1.3.3 | E192K | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
4.1.3.3 | E192L | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
4.1.3.3 | E192M | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
4.1.3.3 | E192N | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
4.1.3.3 | E192P | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
4.1.3.3 | E192Q | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
4.1.3.3 | E192R | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
4.1.3.3 | E192S | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
4.1.3.3 | E192T | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
4.1.3.3 | E192V | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
4.1.3.3 | E192W | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
4.1.3.3 | E192Y | strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.3.3 | (2R,3R)-2,3,4-trihydroxy-N,N-dipropylbutanamide | competitive inhibitor | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.3.3 | 0.2 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192M mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.2 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192P mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.3 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192F mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.4 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192N mutant protein, pH 7.7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.4 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192V mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.5 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192H mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.6 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192S mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.7 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192Q mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 1.1 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192D mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 3.5 | - |
N-acetylneuraminic acid | E192Q mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 4.4 | - |
N-acetylneuraminic acid | wild type (E192), pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 4.6 | - |
N-acetylneuraminic acid | E192D mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 5 | - |
N-acetylneuraminic acid | E192M mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 5.9 | - |
N-acetylneuraminic acid | E192F mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 6 | - |
N-acetylneuraminic acid | E192P mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 6 | - |
N-acetylneuraminic acid | E192S mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 6.2 | - |
N-acetylneuraminic acid | E192H mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 11 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | wild type (E192), pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 34 | - |
N-acetylneuraminic acid | E192V mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 38 | - |
N-acetylneuraminic acid | E192N mutant protein, pH 7.7.5, 25°C | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.3.3 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.3.3 | immobilized metal ion affinity chromatography (Ni2+), gel filtration | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.3.3 | (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | - |
Escherichia coli | pyruvate + (2R,3S)-2,3-dihydroxy-4-oxo-N,N-dipropylbutanamide | - |
r | |
4.1.3.3 | N-acetylmannosamine + pyruvate | - |
Escherichia coli | N-acetylneuraminic acid | - |
r | |
4.1.3.3 | N-acetylneuraminic acid | - |
Escherichia coli | N-acetylmannosamine + pyruvate | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.3.3 | N-Acetylneuraminic acid aldolase | - |
Escherichia coli |
4.1.3.3 | N-Acetylneuraminic acid lyase | - |
Escherichia coli |
4.1.3.3 | Sialic acid aldolase | - |
Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.3.3 | 0.1 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192D mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.42 | - |
N-acetylneuraminic acid | E192P mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.43 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192S mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.63 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192H mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.82 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192P mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.83 | - |
N-acetylneuraminic acid | E192S mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 1.17 | - |
N-acetylneuraminic acid | E192M mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 1.17 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192M mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 1.22 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | wild type (E192), pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 1.47 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192F mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 1.58 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192V mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 2 | - |
N-acetylneuraminic acid | E192D mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 2 | - |
N-acetylneuraminic acid | E192H mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 2 | - |
N-acetylneuraminic acid | E192V mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 2.17 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192N mutant protein, pH 7.7.5, 25°C | Escherichia coli | |
4.1.3.3 | 2.67 | - |
N-acetylneuraminic acid | E192F mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 2.83 | - |
N-acetylneuraminic acid | E192N mutant protein, pH 7.7.5, 25°C | Escherichia coli | |
4.1.3.3 | 2.83 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192Q mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 4.33 | - |
N-acetylneuraminic acid | wild type (E192), pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 5 | - |
N-acetylneuraminic acid | E192Q mutant protein, pH 7.5, 25°C | Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.3.3 | 1.3 | - |
(2R,3R)-2,3,4-trihydroxy-N,N-dipropylbutanamide | E192N mutant protein , pH 7.5, 25°C | Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.3.3 | 0.06 | - |
N-acetylneuraminic acid | E192V mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.07 | - |
N-acetylneuraminic acid | E192N mutant protein, pH 7.7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.07 | - |
N-acetylneuraminic acid | E192P mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.08 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192D mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.12 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | wild type (E192), pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.13 | - |
N-acetylneuraminic acid | E192S mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.23 | - |
N-acetylneuraminic acid | E192M mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.32 | - |
N-acetylneuraminic acid | E192H mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.43 | - |
N-acetylneuraminic acid | E192D mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.45 | - |
N-acetylneuraminic acid | E192F mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.7 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192S mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 0.98 | - |
N-acetylneuraminic acid | wild type (E192), pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 1.33 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192H mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 1.42 | - |
N-acetylneuraminic acid | E192Q mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 4.17 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192Q mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 4.33 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192P mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 4.5 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192V mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 5.17 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192F mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 5.33 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192M mutant protein, pH 7.5, 25°C | Escherichia coli | |
4.1.3.3 | 5.67 | - |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid | E192N mutant protein, pH 7.7.5, 25°C | Escherichia coli |