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Literature summary extracted from

  • Partha, S.K.; Sadeghi-Khomami, A.; Slowski, K.; Kotake, T.; Thomas, N.R.; Jakeman, D.L.; Sanders, D.A.
    Chemoenzymatic synthesis, inhibition studies, and X-ray crystallographic analysis of the phosphono analog of UDP-Galp as an inhibitor and mechanistic probe for UDP-galactopyranose mutase (2010), J. Mol. Biol., 403, 578-590.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.9 expressed in Escherichia coli C43 cells Klebsiella pneumoniae
5.4.99.9 expressed in Escherichia coli C43 cells Mycobacterium tuberculosis
5.4.99.9 expressed in Escherichia coli C43 cells Deinococcus radiodurans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.99.9 in complex with UDP-CH2-Galp, hanging drop vapor diffusion method, using 0.1 M HEPES (pH 6.5), 0.2 M LiCl, and 28% (w/v) polyethylene glycol 6000 Deinococcus radiodurans

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.9 UDP-CH2-Galp moderate inhibition of UGM Deinococcus radiodurans
5.4.99.9 UDP-CH2-Galp moderate inhibition of UGM Klebsiella pneumoniae
5.4.99.9 UDP-CH2-Galp moderate inhibition of UGM Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.9 Deinococcus radiodurans Q9RYF1
-
-
5.4.99.9 Klebsiella pneumoniae
-
-
-
5.4.99.9 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.9 Protino Ni-IDA 1000 column chromatography Klebsiella pneumoniae
5.4.99.9 Protino Ni-IDA 1000 column chromatography Mycobacterium tuberculosis
5.4.99.9 Protino Ni-IDA 1000 column chromatography Deinococcus radiodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.9 UDP-alpha-D-galactopyranose
-
 Klebsiella pneumoniae UDP-alpha-D-galactofuranose
-
 r
5.4.99.9 UDP-alpha-D-galactopyranose
-
 Mycobacterium tuberculosis UDP-alpha-D-galactofuranose
-
 r
5.4.99.9 UDP-alpha-D-galactopyranose
-
 Deinococcus radiodurans UDP-alpha-D-galactofuranose
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.4.99.9 galactopyranose mutase
-
 Klebsiella pneumoniae
5.4.99.9 galactopyranose mutase
-
 Mycobacterium tuberculosis
5.4.99.9 galactopyranose mutase
-
 Deinococcus radiodurans
5.4.99.9 UGM
-
 Klebsiella pneumoniae
5.4.99.9 UGM
-
 Mycobacterium tuberculosis
5.4.99.9 UGM
-
 Deinococcus radiodurans

Cofactor

EC Number Cofactor Comment Organism Structure
5.4.99.9 FAD
-
 Klebsiella pneumoniae
5.4.99.9 FAD
-
 Mycobacterium tuberculosis
5.4.99.9 FAD
-
 Deinococcus radiodurans

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5.4.99.9 0.411
-
 in 50 mM sodium phosphate buffer (pH 7.0), at 22°C Deinococcus radiodurans UDP-CH2-Galp
5.4.99.9 0.479
-
 in 50 mM sodium phosphate buffer (pH 7.0), at 22°C Klebsiella pneumoniae UDP-CH2-Galp
5.4.99.9 0.495
-
 in 50 mM sodium phosphate buffer (pH 7.0), at 22°C Mycobacterium tuberculosis UDP-CH2-Galp