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Literature summary extracted from

  • Cherney, M.M.; Zhang, Y.; Solomonson, M.; Weiner, J.H.; James, M.N.
    Crystal structure of sulfide:quinone oxidoreductase from Acidithiobacillus ferrooxidans: insights into sulfidotrophic respiration and detoxification (2010), J. Mol. Biol., 398, 292-305.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.5.4
-
 Acidithiobacillus ferrooxidans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.5.4 native or in complex with decylubiquinone, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol 600, 0.1 M 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol (pH 5.5 or pH 6.5), 0.1 M magnesium sulfate, and 0.05% (w/v) n-dodecyl beta-D-maltoside Acidithiobacillus ferrooxidans

Protein Variants

EC Number Protein Variants Comment Organism
1.8.5.4 C128A in the decylubiquinone assay, the mutant shows 30-35% activity compared to the wild type enzyme. However, in the FAD reduction assay, both the wild type and the Cys128Ala variant are fully active (100%) Acidithiobacillus ferrooxidans
1.8.5.4 C160A inactive Acidithiobacillus ferrooxidans
1.8.5.4 C356A inactive Acidithiobacillus ferrooxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.5.4 0.0028
-
 Sulfide in 50 mM 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol (pH 6.5), temperature not specified in the publication Acidithiobacillus ferrooxidans
1.8.5.4 0.022
-
 decylubiquinone in 50 mM 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol (pH 6.5), temperature not specified in the publication Acidithiobacillus ferrooxidans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.5.4 membrane
-
 Acidithiobacillus ferrooxidans 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.4 Acidithiobacillus ferrooxidans B7JBP8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.5.4
-
 Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.4 additional information the enzymatic reaction catalyzed by sulfide:quinone oxidoreductase includes the oxidation of sulfide compounds H2S, HS-, and S2- to soluble polysulfide chains or to elemental sulfur in the form of octasulfur rings Acidithiobacillus ferrooxidans ?
-
 ?
1.8.5.4 sulfide + decylubiquinone
-
 Acidithiobacillus ferrooxidans sulfur + decylubiquinol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.5.4 dimer x-ray crystallography Acidithiobacillus ferrooxidans

Synonyms

EC Number Synonyms Comment Organism
1.8.5.4 sulfide:quinone oxidoreductase
-
 Acidithiobacillus ferrooxidans

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.5.4 FAD noncovalently bound Acidithiobacillus ferrooxidans