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Literature summary extracted from

  • Zha, M.; Zhong, C.; Peng, Y.; Hu, H.; Ding, J.
    Crystal structures of human NUDT5 reveal insights into the structural basis of the substrate specificity (2006), J. Mol. Biol., 364, 1021-1033.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.13 expressed in Escherichia coli BL21(DE3) cells Homo sapiens
3.6.1.58
-
 Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.13 in apo form, in complex with ADP-D-ribose, and in complex with AMP with bound Mg2+, hanging drop vapor diffusion method, using 160 mM sodium acetate (pH 5.5), and 25% (w/v) 2-methyl-2,4-pentanediol, or 300 mM di-ammonium hydrogen citrate, 6% (w/v) n-propanol and 15% (w/v) polyethylene glycol 3350, or 200 mM sodium acetate, 100 mM Tris-HCl (pH 8.0) and 30% (w/v) polyethylene glycol 4000 Homo sapiens
3.6.1.58 crystals of full-length hNUDT5 are grown at 4°C using the hanging-drop vapor diffusion method. Crystal structures of hNUDT5 in apo form, in complex with ADP-ribose, and in complex with AMP with bound Mg2+ Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.13 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.13 ADP-D-ribose + H2O Homo sapiens
-
 AMP + D-ribose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.13 Homo sapiens Q9UKK9
-
-
3.6.1.58 Homo sapiens Q9UKK9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.13 Ni-NTA column chromatography and Q-Sepharose column chromatography Homo sapiens
3.6.1.58
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.13 ADP-D-ribose + H2O
-
 Homo sapiens AMP + D-ribose 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.13 homodimer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.6.1.13 ADP-ribose pyrophosphatase
-
 Homo sapiens
3.6.1.13 ADPRase
-
 Homo sapiens
3.6.1.13 NUDT5
-
 Homo sapiens
3.6.1.58 NUDT5
-
 Homo sapiens