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Literature summary extracted from
- Isolation, Purification, and enzymatic characterization of extracellular chitosanase from marine bacterium Bacillus subtilis CH2 (2011), J. Microbiol. Biotechnol., 21, 1021-1025.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.132 | 29000 | - |
x * 29000, SDS-PAGE | Bacillus subtilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.132 | Bacillus subtilis | - |
- |
- |
| 3.2.1.132 | Bacillus subtilis CH2 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.132 | ammonium sulfate precipitation and Sephadex-G100 gel filtration | Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.132 | chitosan + H2O | - |
Bacillus subtilis | ? | - |
? |  |
| 3.2.1.132 | chitosan + H2O | - |
Bacillus subtilis CH2 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.132 | ? | x * 29000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.132 | 60 | - |
- |
Bacillus subtilis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.132 | 40 | 60 | the purified chitosanase is continuously thermostable at 40°C and the stability is gradually decreased when the temperature is increased to 50°C but rapidly decreases at 60°C | Bacillus subtilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.132 | 5.5 | - |
- |
Bacillus subtilis |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.2.1.132 | Bacillus subtilis | enzyme expression is not induced using chitosan as a carbon source | additional information |
| 3.2.1.132 | Bacillus subtilis | the highest chitosanase activity is observed with fructose, and it shows high activity with other substrates such as lactose, galactose, maltose, and glucose as well | up |
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