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Literature summary extracted from

  • Kim, Y.A.; Yoon, K.H.
    Characterization of a Paenibacillus woosongensis beta-xylosidase/alpha-arabinofuranosidase produced by recombinant Escherichia coli (2010), J. Microbiol. Biotechnol., 20, 1711-1716.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.55 SDS SDS (5 mM) enhances the enzymatic activity for 4-nitrophenyl alpha-L-arabinofuranoside by 12% Paenibacillus woosongensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.37 expression in Escherichia coli Paenibacillus woosongensis
3.2.1.55 expressed in Escherichia coli BL21(DE3) Codon Plus cells Paenibacillus woosongensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 EDTA 5 mM, 79% residual activity with substrate 4-nitrophenyl beta-D-xylopyranoside, 68% activity with substrate 4-nitrophenyl alpha-L-arabinofuranoside Paenibacillus woosongensis
3.2.1.37 Fe2+ 5 mM, 57% residual activity with substrate 4-nitrophenyl beta-D-xylopyranoside, 12% activity with substrate 4-nitrophenyl alpha-L-arabinofuranoside Paenibacillus woosongensis
3.2.1.37 Fe3+ 5 mM, 170% residual activity with substrate 4-nitrophenyl beta-D-xylopyranoside, 49% activity with substrate 4-nitrophenyl alpha-L-arabinofuranoside Paenibacillus woosongensis
3.2.1.37 SDS 5 mM, 70% residual activity with substrate 4-nitrophenyl beta-D-xylopyranoside, 112% activity with substrate 4-nitrophenyl alpha-L-arabinofuranoside Paenibacillus woosongensis
3.2.1.55 Cu2+ 88% residual activity at 5 mM Paenibacillus woosongensis
3.2.1.55 EDTA 68% residual activity at 5 mM Paenibacillus woosongensis
3.2.1.55 Fe2+ 12% residual activity at 5 mM Paenibacillus woosongensis
3.2.1.55 Fe3+ 49% residual activity at 5 mM Paenibacillus woosongensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.37 Fe3+ 5 mM, 170% residual activity with substrate 4-nitrophenyl beta-D-xylopyranoside, 49% activity with substrate 4-nitrophenyl alpha-L-arabinofuranoside Paenibacillus woosongensis
3.2.1.37 SDS 5 mM, 70% residual activity with substrate 4-nitrophenyl beta-D-xylopyranoside, 112% activity with substrate 4-nitrophenyl alpha-L-arabinofuranoside Paenibacillus woosongensis
3.2.1.55 Ca2+ 106% activity at 5 mM Paenibacillus woosongensis
3.2.1.55 K+ 107% activity at 5 mM Paenibacillus woosongensis
3.2.1.55 Mg2+ 145% activity at 5 mM Paenibacillus woosongensis
3.2.1.55 Mn2+ 133% activity at 5 mM Paenibacillus woosongensis
3.2.1.55 Ni2+ 109% activity at 5 mM Paenibacillus woosongensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 52852
-
 x * 52852, calculated from amino acid sequence Paenibacillus woosongensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Paenibacillus woosongensis E7DXB6 bifunctional beta-xylosidase/alpha-arabinofuranosidase
-
3.2.1.37 Paenibacillus woosongensis DSM 16971 E7DXB6 bifunctional beta-xylosidase/alpha-arabinofuranosidase
-
3.2.1.55 Paenibacillus woosongensis E7DXB6
-
-
3.2.1.55 Paenibacillus woosongensis KCTC 3953 (DSM 16971) E7DXB6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 recombinasnt protein Paenibacillus woosongensis
3.2.1.55 Ni-NTA column chromatography Paenibacillus woosongensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.37 347.3
-
 substrate 4-nitrophenyl alpha-L-arabinofuranoside, pH 7.0, 45°C Paenibacillus woosongensis
3.2.1.37 659.9
-
 substrate 4-nitrophenyl beta-D-xylopyranoside, pH 6.5, 50°C Paenibacillus woosongensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Paenibacillus woosongensis 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.37 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Paenibacillus woosongensis DSM 16971 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.37 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Paenibacillus woosongensis KCTC 3953 (DSM 16971) 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Paenibacillus woosongensis 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Paenibacillus woosongensis DSM 16971 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Paenibacillus woosongensis KCTC 3953 (DSM 16971) 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Paenibacillus woosongensis 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Paenibacillus woosongensis DSM 16971 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Paenibacillus woosongensis KCTC 3953 (DSM 16971) 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 additional information XylC does use 4-nitrophenyl beta-xylopyranoside as substrate with about 2fold higher specific activity than 4-nitrophenyl alpha-L-arabinofuranoside. The relative enzyme activity is approximately 52% for 4-nitrophenyl beta-xylopyranoside and 76% for 4-nitrophenyl alpha-L-arabinofuranoside at pH 8.0. The enzyme shows very low activity against 4-nitrophenyl alpha-L-arabinopyranoside and 4-nitrophenyl beta-D-galactopyranoside Paenibacillus woosongensis ?
-
 ?
3.2.1.55 additional information XylC does use 4-nitrophenyl beta-xylopyranoside as substrate with about 2fold higher specific activity than 4-nitrophenyl alpha-L-arabinofuranoside. The relative enzyme activity is approximately 52% for 4-nitrophenyl beta-xylopyranoside and 76% for 4-nitrophenyl alpha-L-arabinofuranoside at pH 8.0. The enzyme shows very low activity against 4-nitrophenyl alpha-L-arabinopyranoside and 4-nitrophenyl beta-D-galactopyranoside Paenibacillus woosongensis DSM 16971 ?
-
 ?
3.2.1.55 additional information XylC does use 4-nitrophenyl beta-xylopyranoside as substrate with about 2fold higher specific activity than 4-nitrophenyl alpha-L-arabinofuranoside. The relative enzyme activity is approximately 52% for 4-nitrophenyl beta-xylopyranoside and 76% for 4-nitrophenyl alpha-L-arabinofuranoside at pH 8.0. The enzyme shows very low activity against 4-nitrophenyl alpha-L-arabinopyranoside and 4-nitrophenyl beta-D-galactopyranoside Paenibacillus woosongensis KCTC 3953 (DSM 16971) ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 ? x * 53000-54000, SDS-PAGE Paenibacillus woosongensis
3.2.1.55 ? x * 52852, calculated from amino acid sequence Paenibacillus woosongensis
3.2.1.55 ? x * 53000-54000, estimated from SDS-PAGE Paenibacillus woosongensis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 XylC
-
 Paenibacillus woosongensis
3.2.1.55 beta-xylosidase/alpha-arabinofuranosidase bifunctional enzyme Paenibacillus woosongensis
3.2.1.55 XylC bifunctional enzyme Paenibacillus woosongensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 45
-
 substrate 4-nitrophenyl alpha-L-arabinofuranoside Paenibacillus woosongensis
3.2.1.37 50
-
 substrate 4-nitrophenyl beta-D-xylopyranoside Paenibacillus woosongensis
3.2.1.55 50
-
-
 Paenibacillus woosongensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 30
-
 3 h, stable Paenibacillus woosongensis
3.2.1.37 40
-
 3 h, 83% residual activity Paenibacillus woosongensis
3.2.1.37 45
-
 rapid decrease in activity above Paenibacillus woosongensis
3.2.1.55 30 45 the enzyme is stable up to 30°C for 3 h, and it retains 83% of its maximal activity at 40°C after 3 h of incubation. However, the stability of the enzyme decreases rapidly at temperature above 45°C Paenibacillus woosongensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 6.5
-
 substrate 4-nitrophenyl beta-D-xylopyranoside Paenibacillus woosongensis
3.2.1.37 7
-
 substrate 4-nitrophenyl alpha-L-arabinofuranoside Paenibacillus woosongensis
3.2.1.55 6.5
-
-
 Paenibacillus woosongensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.37 6 7 more than 80% of maximum activity within Paenibacillus woosongensis
3.2.1.55 6 7 more than 75% activity between pH 6.0 and 7.0 Paenibacillus woosongensis