EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.91 | cellobiose | competitive inhibition | Fomitopsis pinicola | |
3.2.1.91 | Hg2+ | 60% inhibition at 1 mM | Fomitopsis pinicola | |
3.2.1.91 | additional information | the enzyme is neither inhibited nor activated by EDTA at concentrations ranging from 1 to 10 mM | Fomitopsis pinicola | |
3.2.1.91 | N-ethylmaleimide | 30% inhibition at 10 mM | Fomitopsis pinicola |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.91 | 2.1 | - |
4-nitrophenyl beta-D-cellobioside | in 100 mM sodium acetate buffer (pH 5.0) at 50°C | Fomitopsis pinicola |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.91 | additional information | enzyme activity is not stimulated by Ba2+, Mg2+, Mn2+, Zn2+, Ca2+, Ni2+, or Co2+ (each at 1 mM) | Fomitopsis pinicola |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.91 | 64000 | - |
gel filtration | Fomitopsis pinicola |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.91 | Fomitopsis pinicola | - |
- |
- |
3.2.1.91 | Fomitopsis pinicola KMJ812 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.91 | DEAE Sepharose column chromatography, Sephacryl S-300 gel filtration, and Mono Q column chromatography | Fomitopsis pinicola |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.91 | culture condition:peptone-grown cell | - |
Fomitopsis pinicola | - |
3.2.1.91 | culture condition:rice straw-grown cell | - |
Fomitopsis pinicola | - |
3.2.1.91 | culture condition:yeast extract-grown cell | - |
Fomitopsis pinicola | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.91 | 4 | - |
crude cell extract, using 4-nitrophenyl beta-D-cellobioside as substrate, at pH 5.0, 50°C | Fomitopsis pinicola |
3.2.1.91 | 26 | - |
after 6.8fold purification, using 4-nitrophenyl beta-D-cellobioside as substrate, at pH 5.0, 50°C | Fomitopsis pinicola |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.91 | 4-nitrophenyl beta-D-cellobioside + H2O | 100% activity, highly preferred substrate | Fomitopsis pinicola | 4-nitrophenol + cellobiose | - |
? | |
3.2.1.91 | 4-nitrophenyl beta-D-galactopyranoside + H2O | 0.26% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | 4-nitrophenol + D-galactopyranose | - |
? | |
3.2.1.91 | 4-nitrophenyl beta-D-glucopyranoside + H2O | 0.11% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | 4-nitrophenol + D-glucopyranose | - |
? | |
3.2.1.91 | 4-nitrophenyl beta-D-glucopyranoside + H2O | 0.11% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola KMJ812 | 4-nitrophenol + D-glucopyranose | - |
? | |
3.2.1.91 | 4-nitrophenyl beta-D-lactoside + H2O | 42.1% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | 4-nitrophenol + lactose | - |
? | |
3.2.1.91 | 4-nitrophenyl beta-D-mannopyranoside + H2O | 0.64% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | 4-nitrophenol + D-mannopyranose | - |
? | |
3.2.1.91 | 4-nitrophenyl beta-D-xylopyranoside + H2O | 0.64% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | 4-nitrophenol + D-xylopyranose | - |
? | |
3.2.1.91 | avicel + H2O | 26.9% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | ? | - |
? | |
3.2.1.91 | carboxymethylcellulose + H2O | 0.26% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | ? | - |
? | |
3.2.1.91 | cellobiose + H2O | 0.11% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | ? | - |
? | |
3.2.1.91 | laminarin + H2O | 1.5% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | ? | - |
? | |
3.2.1.91 | laminarin + H2O | 1.5% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola KMJ812 | ? | - |
? | |
3.2.1.91 | lichenan + H2O | 33.8% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | ? | - |
? | |
3.2.1.91 | lichenan + H2O | 33.8% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola KMJ812 | ? | - |
? | |
3.2.1.91 | additional information | no activity with cellotriose, and cellotetraose | Fomitopsis pinicola | ? | - |
? | |
3.2.1.91 | additional information | no activity with cellotriose, and cellotetraose | Fomitopsis pinicola KMJ812 | ? | - |
? | |
3.2.1.91 | xylan + H2O | 1.13% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | ? | - |
? | |
3.2.1.91 | xylan + H2O | 1.13% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola KMJ812 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.91 | monomer | 1 * 64000, SDS-PAGE | Fomitopsis pinicola |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.91 | CBH | - |
Fomitopsis pinicola |
3.2.1.91 | cellobiohydrolase | - |
Fomitopsis pinicola |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.91 | 50 | - |
- |
Fomitopsis pinicola |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.91 | 45 | 55 | 80% and 95% of the maximum activity appearing at 45°C and 55°C | Fomitopsis pinicola |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.91 | 40 | 70 | the enzyme maintains about 90% activity when incubated at 60°C for 24 h. About 90% activity is maintained after 8 h of incubation at 70°C. At temperatures over 80°C, the enzyme activity sharply decreases depending on the incubation time. The enzyme shows half-life values of 552 h, 144 h, 96 h, and 42 h at 40°C, 50°C, 60°C, and 70°C, respectively | Fomitopsis pinicola |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.91 | 5 | - |
- |
Fomitopsis pinicola |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.91 | 4.5 | 5.5 | 90% and 93% of the maximum activity appearing at pH 4.5 and 5.5 | Fomitopsis pinicola |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.91 | 14.8 | - |
cellobiose | at pH 5.0, 50°C | Fomitopsis pinicola |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.91 | Fomitopsis pinicola | isoelectric focusing | - |
5.1 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.91 | 15.8 | - |
4-nitrophenyl beta-D-cellobioside | in 100 mM sodium acetate buffer (pH 5.0) at 50°C | Fomitopsis pinicola |