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Literature summary extracted from

  • Simila, J.; Gernig, A.; Murray, P.; Fernandes, S.; Tuohy, M.G.
    Cloning and expression of a thermostable alpha-galactosidase from the thermophilic fungus Talaromyces emersonii in the methylotrophic yeast Pichia pastoris (2010), J. Microbiol. Biotechnol., 20, 1653-1663.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.22 expressed in Pichia pastoris as a His-tagged fusion protein Rasamsonia emersonii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 galactose
-
 Rasamsonia emersonii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.29
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 4.5, 50°C Rasamsonia emersonii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 75000
-
 SDS-PAGE after treatment with endoglycosidase, recombinant protein Rasamsonia emersonii
3.2.1.22 110000
-
 SDS-PAGE, recombinant protein Rasamsonia emersonii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Rasamsonia emersonii
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.22 glycoprotein
-
 Rasamsonia emersonii

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22 using Ni-NTA chromatography Rasamsonia emersonii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-nitrophenyl-alpha-D-galactopyranoside + H2O
-
 Rasamsonia emersonii 4-nitrophenol + alpha-D-galactopyranose
-
 ?
3.2.1.22 carob galactomannan + H2O
-
 Rasamsonia emersonii ?
-
 ?
3.2.1.22 di-galactomannopentose + H2O
-
 Rasamsonia emersonii ?
-
 ?
3.2.1.22 galactomannotriose + H2O
-
 Rasamsonia emersonii ?
-
 ?
3.2.1.22 guar gum + H2O
-
 Rasamsonia emersonii ?
-
 ?
3.2.1.22 locust bean gum + H2O
-
 Rasamsonia emersonii ?
-
 ?
3.2.1.22 melibiose + H2O
-
 Rasamsonia emersonii alpha-D-galactose + alpha-D-glucose
-
 ?
3.2.1.22 raffinose + H2O
-
 Rasamsonia emersonii sucrose + alpha-D-galactose
-
 ?
3.2.1.22 stachyose + 2 H2O
-
 Rasamsonia emersonii 2 alpha-D-galactose + sucrose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 monomer
-
 Rasamsonia emersonii

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 alpha-gal1
-
 Rasamsonia emersonii
3.2.1.22 alpha-galactosidase
-
 Rasamsonia emersonii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 70
-
-
 Rasamsonia emersonii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.22 60 70
-
 Rasamsonia emersonii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 50
-
 no loss in activity after 10 days at 50°C Rasamsonia emersonii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 4.5
-
-
 Rasamsonia emersonii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.22 2.77
-
 galactose pH 4.5, 50°C Rasamsonia emersonii