Literature summary extracted from

Beena, P.S.; Soorej, M.B.; Elyas, K.K.; Sarita, G.B.; Chandrasekaran, M.
Acidophilic tannase from marine Aspergillus awamori BTMFW032 (2010), J. Microbiol. Biotechnol., 20, 1403-1414.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
3.1.1.20	dithiothreitol	enhances activity at low concentrations of 0.2% (v/v) (108.1% residual activity) to 0.6% (v/v) (111.8% residual activity)	Aspergillus awamori
3.1.1.20	Sodium thioglycolate	an increase in residual enzyme activity from 23.1% (0.2% concentration) to a maximum of 321.2% (1.0% concentration) is noted along with an increase in its concentration	Aspergillus awamori

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.1.1.20	Al3+	complete inhibition at 1 mM	Aspergillus awamori
3.1.1.20	Ba2+	complete inhibition at 5 mM	Aspergillus awamori
3.1.1.20	beta-mercaptoethanol	-	Aspergillus awamori
3.1.1.20	Ca2+	0.84% residual activity at 20 mM	Aspergillus awamori
3.1.1.20	Cd2+	11.82% residual activity at 20 mM	Aspergillus awamori
3.1.1.20	Co2+	39.06% residual activity at 20 mM	Aspergillus awamori
3.1.1.20	Cu2+	10.58% residual activity at 20 mM	Aspergillus awamori
3.1.1.20	dithiothreitol	displays inhibitory properties at higher concentrations of 1.0% to 5.0% (v/v)	Aspergillus awamori
3.1.1.20	H2O2	H2O2 concentrations of less than 2% and higher than 10% lead to a decline in enzyme activity	Aspergillus awamori
3.1.1.20	Hg2+	53.02% residual activity at 20 mM	Aspergillus awamori
3.1.1.20	Li+	3.07% residual activity at 20 mM	Aspergillus awamori
3.1.1.20	Mg2+	7.03% residual activity at 20 mM	Aspergillus awamori
3.1.1.20	Mn2+	10.18% residual activity at 20 mM	Aspergillus awamori
3.1.1.20	Mo2+	22.48% residual activity at 20 mM	Aspergillus awamori
3.1.1.20	Zn2+	30.54% residual activity at 20 mM	Aspergillus awamori

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.1.1.20	1.9	-	methyl gallate	at pH 2.0, 30°C	Aspergillus awamori

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.1.1.20	extracellular	-	Aspergillus awamori	-	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
3.1.1.20	Fe3+	144.44% activity at 10 mM	Aspergillus awamori
3.1.1.20	K+	176.62% activity at 10 mM	Aspergillus awamori
3.1.1.20	Na+	120.37% activity at 20 mM	Aspergillus awamori

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.1.1.20	37800	-	6 * 37800, SDS-PAGE	Aspergillus awamori
3.1.1.20	230000	-	gel filtration	Aspergillus awamori

Organic Solvent Stability

EC Number	Organic Solvent	Comment	Organism
3.1.1.20	Acetone	about 70% activity after 1 h at 10% (v/v)	Aspergillus awamori
3.1.1.20	acetonitrile	about 85% activity after 1 h at 10% (v/v)	Aspergillus awamori
3.1.1.20	benzene	about 45% activity after 1 h at 10% (v/v)	Aspergillus awamori
3.1.1.20	Brij-35	the enzyme is not stable in the presence of Brij-35, with less than 82% residual activity at 0.1-1% (v/v)	Aspergillus awamori
3.1.1.20	Butanol	about 70% activity after 1 h at 10% (v/v)	Aspergillus awamori
3.1.1.20	chloroform	about 60% activity after 1 h at 10% (v/v)	Aspergillus awamori
3.1.1.20	DMSO	at a 60% concentration of DMSO, the enzyme retains a 61% activity after 24 h	Aspergillus awamori
3.1.1.20	Ethanol	about 80% activity after 1 h at 10% (v/v)	Aspergillus awamori
3.1.1.20	hexane	about 80% activity after 1 h at 10% (v/v)	Aspergillus awamori
3.1.1.20	isopropanol	about 70% activity after 1 h at 10% (v/v)	Aspergillus awamori
3.1.1.20	Methanol	about 80% activity after 1 h at 10% (v/v)	Aspergillus awamori
3.1.1.20	Triton X-100	at high concentrations of between 0.6% (v/v) (79.06% residual activity) to 1.0% (v/v) (54.26% residual activity), lead to a loss of enzyme stability, although the enzyme is stable at a 0.2% (v/v) concentration (112.2% residual activity) with an enhanced enzyme activity at a 0.4% concentration (171.36% residual activity)	Aspergillus awamori
3.1.1.20	Tween 80	Tween 80 leads to an enhanced enzyme activity at up to a 0.6% (v/v) concentration (157.89% residual activity), but displays inhibitory properties at a high concentration of 1.0% (v/v) (83.5% residual activity)	Aspergillus awamori

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.1.20	Aspergillus awamori	C7F6Y1	-	-
3.1.1.20	Aspergillus awamori BTMFW032	C7F6Y1	-	-

Oxidation Stability

EC Number	Oxidation Stability	Organism
3.1.1.20	increases in hydrogen peroxide concentrations result in enhanced enzyme activity in the range of 2% (161.1% activity) to 10% (220% activity)	Aspergillus awamori

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.1.1.20	ultrafiltration and Sephadex G-200 gel filtration	Aspergillus awamori

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.1.1.20	436.7	-	crude extract, pH 2.0, 30°C	Aspergillus awamori
3.1.1.20	2762	-	after 6.32fold purification, pH 2.0, 30°C	Aspergillus awamori

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.1.20	methyl gallate + H2O	best substrate	Aspergillus awamori	gallate + methanol	-	?
3.1.1.20	methyl gallate + H2O	best substrate	Aspergillus awamori BTMFW032	gallate + methanol	-	?
3.1.1.20	propyl gallate + H2O	-	Aspergillus awamori	gallate + propanol	-	?
3.1.1.20	propyl gallate + H2O	-	Aspergillus awamori BTMFW032	gallate + propanol	-	?
3.1.1.20	tannic acid + H2O	lowest activity	Aspergillus awamori	gallic acid + D-glucose	-	?
3.1.1.20	tannic acid + H2O	lowest activity	Aspergillus awamori BTMFW032	gallic acid + D-glucose	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.1.1.20	homohexamer	6 * 37800, SDS-PAGE	Aspergillus awamori

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.1.20	tannin acyl hydrolase	-	Aspergillus awamori

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
3.1.1.20	30	-	-	Aspergillus awamori

Temperature Range [°C]

EC Number	Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
3.1.1.20	5	80	the enzyme activity declines markedly along with increases in temperature above 60°C	Aspergillus awamori

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
3.1.1.20	30	70	the enzyme is stable only at 30°C for 24 h, retaining about 60% activity. The residual activity is 72% at 40°C for 2 h, 54% at 50°C for 6 h, 53% at 60°C for 4 h, and 72% at 70°C for 1 h	Aspergillus awamori

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.1.1.20	2	-	two pH optima, pH 2.0 and pH 8.0, are recorded	Aspergillus awamori
3.1.1.20	8	-	two pH optima, pH 2.0 and pH 8.0, are recorded	Aspergillus awamori

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
3.1.1.20	1	7	the enzyme records a significant level of activity even at a pH of 1.0, however, the enzyme activity declines rapidly at pH 3.0-4.0 and is almost nil at a pH of 7.0	Aspergillus awamori

pH Stability

EC Number	pH Stability	pH Stability Maximum	Comment	Organism
3.1.1.20	2	-	the enzyme is stable (99%) over a relatively long duration of incubation (up to 24 h) only at pH 2.0. At other pH levels, the enzyme is not stable	Aspergillus awamori

pI Value

EC Number	Organism	Comment	pI Value Maximum	pI Value
3.1.1.20	Aspergillus awamori	isoelectric focusing	-	4.4

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Release 2023.1 (January 2023)