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Literature summary extracted from

  • Basu, D.; Manjur, J.; Jin, W.
    Determination of lipoprotein lipase activity using a novel fluorescent lipase assay (2011), J. Lipid Res., 52, 826-832.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.34 apoC-II apoC-II activates the enzyme 3.5fold in a saturable fashion. Heat-inactivated rat serum is often used as a source of apoC-II for activation of lipoprotein lipase Mus musculus
3.1.1.34 apoC-II apoC-II activates the enzyme 3.5fold in a saturable fashion. Heat-inactivated rat serum is often used as a source of apoC-II for activation of lipoprotein lipase Bos taurus
3.1.1.34 heparin addition of heparin increases enzyme activity minimally by about 6% Mus musculus
3.1.1.34 heparin addition of heparin increases enzyme activity minimally by about 6% Bos taurus
3.1.1.34 NaCl addition of NaCl increases the reaction rate with EnzChek lipase substrate dramatically with the highest rate, 46% higher than that without salt, occurring at 0.15 M Mus musculus
3.1.1.34 NaCl addition of NaCl increases the reaction rate with EnzChek lipase substrate dramatically with the highest rate, 46% higher than that without salt, occurring at 0.15 M Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.34 angiopoietin-like protein-4
-
 Bos taurus
3.1.1.34 angiopoietin-like protein-4
-
 Mus musculus
3.1.1.34 heat-inactivated rat serum heat-inactivated rat serum added from 0-10%, decreases the enzyme activity by 12%. HIS also contains lipoprotein lipase-inhibitory factors such as angiopoietin-like protein-3, angiopoietin-like protein-4, apoC-I, and apoC-III Bos taurus
3.1.1.34 heat-inactivated rat serum heat-inactivated rat serum added from 0-10%, decreases the enzyme activity by 12%. HIS also contains lipoprotein lipase-inhibitory factors such as angiopoietin-like protein-3, angiopoietin-like protein-4, apoC-I, and apoC-III Mus musculus
3.1.1.34 NaCl 1 M NaCl inhibits the reaction with triolein by 80%, but there is no inhibition of lipoprotein lipase activity by NaCl if apoC-II is not used in the assay Bos taurus
3.1.1.34 NaCl 1 M NaCl inhibits the reaction with triolein by 80%, but there is no inhibition of lipoprotein lipase activity by NaCl if apoC-II is not used in the assay Mus musculus
3.1.1.34 tetrahydrolipstatin active-site inhibitor Bos taurus
3.1.1.34 tetrahydrolipstatin active-site inhibitor Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.34 Ca2+ addition of calcium increases enzyme activity minimally by about 6% Mus musculus
3.1.1.34 Ca2+ addition of calcium increases enzyme activity minimally by about 6% Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.34 Bos taurus
-
-
-
3.1.1.34 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.34 blood plasma
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.34 1,2-O-dilauryl-DL-glycero-3-glutaric acid-(6-methylresorufin ester) + H2O
-
 Mus musculus ?
-
 ?
3.1.1.34 1,2-O-dilauryl-DL-glycero-3-glutaric acid-(6-methylresorufin ester) + H2O
-
 Bos taurus ?
-
 ?
3.1.1.34 EnzChek lipase substrate + H2O a commercially available BODIPY, Dabcyl-labeled triglyceride analog substrate, C58H85BF2N6O6 Mus musculus ?
-
 ?
3.1.1.34 EnzChek lipase substrate + H2O a commercially available BODIPY, Dabcyl-labeled triglyceride analog substrate, C58H85BF2N6O6 Bos taurus ?
-
 ?
3.1.1.34 triolein + H2O
-
 Mus musculus oleate + diolein
-
 ?
3.1.1.34 triolein + H2O
-
 Bos taurus oleate + diolein
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.34 LPL
-
 Mus musculus
3.1.1.34 LPL
-
 Bos taurus