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Literature summary extracted from

  • Kim, D.; Park, S.Y.; Chung, Y.; Park, J.; Lee, S.; Lee, T.K.
    Biochemical characterization of soluble acid and alkaline invertases from shoots of etiolated pea seedlings (2010), J. Integr. Plant Biol., 52, 536-548.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 CuSO4 acid invertase is inhibited about 50% by 4.8 mM CuSO4. Alkaline invertase is inhibited by 0.6 mMCuSO4 up to 50% Pisum sativum
3.2.1.26 HgCl2 does not affect acid invertase activity, 0.05 mM HgCl2 inhibits alkaline invertase activity by about 50% Pisum sativum
3.2.1.26 Tris-HCl Tris-HCl does not affect acid invertase activity up to 14 mM, 10 mM Tris-HCl inhibits alkaline invertase activity by about 50% Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 1.8
-
 sucrose acid invertase, pH 6.5, 37°C Pisum sativum
3.2.1.26 38.6
-
 sucrose alkaline invertase, pH 6.5, 37°C Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.26 soluble
-
 Pisum sativum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.26 22000
-
 x * 22000 and x * 45000, acid invertase, x * 30000, alkaline invertase, SDS-PAGE Pisum sativum
3.2.1.26 30000
-
 x * 22000 and x * 45000, acid invertase, x * 30000, alkaline invertase, SDS-PAGE Pisum sativum
3.2.1.26 45000
-
 x * 22000 and x * 45000, acid invertase, x * 30000, alkaline invertase, SDS-PAGE Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 both soluble acid and alkaline invertase Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.26 shoot shoot of etiolated seedling Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.26 0.82
-
 pH 6.5, 37°C, alkaline invertase Pisum sativum
3.2.1.26 16.6
-
 pH 6.5, 37°C, acid invertase Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 melizitose + H2O acid invertase, 4.6% of the activity with sucrose, alkaline invertase, 3.1% of the activity with sucrose Pisum sativum ?
-
 ?
3.2.1.26 raffinose + H2O acid invertase, 38.9% of the activity with sucrose, alkaline invertase, 40.0% of the activity with sucrose Pisum sativum beta-D-fructose + melibiose
-
 ?
3.2.1.26 sucrose + H2O
-
 Pisum sativum beta-D-fructose + alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.26 ? x * 22000 and x * 45000, acid invertase, x * 30000, alkaline invertase, SDS-PAGE Pisum sativum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.26 37
-
 both acid and alkaline invertase Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 5.3
-
 acid invertase Pisum sativum
3.2.1.26 7.3
-
 alkaline invertase Pisum sativum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.26 4 7.5 acid invertase Pisum sativum
3.2.1.26 5 9 alkaline invertase Pisum sativum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.26 Pisum sativum isoelectric focusing, alkaline invertase
-
 5.7
3.2.1.26 Pisum sativum isoelectric focusing, acid invertase
-
 7