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Literature summary extracted from

  • Vandock, K.P.; Drummond, C.A.; Smith, S.L.; Fioravanti, C.F.
    Midgut and fatbody mitochondrial transhydrogenase activities during larval-pupal development of the tobacco hornworm, Manduca sexta (2010), J. Insect Physiol., 56, 774-779.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.99.22 mitochondrion
-
 Manduca sexta 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.22 ecdysone + NADPH + H+ + O2 Manduca sexta
-
 20-hydroxyecdysone + NADP+ + H2O
-
 ?
1.14.99.22 additional information Manduca sexta E20-M preferentially uses NADPH in catalyzing ecdysone conversion to the physiologically active molting hormone, 20-hydroxyecdysone ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.22 Manduca sexta
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.99.22 fat body
-
 Manduca sexta
-
1.14.99.22 midgut during the ten-day developmental period preceding the larval-pupal molt, fifth larval instar, onset of wandering behavior and a 50fold increase in ecdysone 20-monooxygenase activity occur Manduca sexta
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.22 ecdysone + NADPH + H+ + O2
-
 Manduca sexta 20-hydroxyecdysone + NADP+ + H2O
-
 ?
1.14.99.22 additional information E20-M preferentially uses NADPH in catalyzing ecdysone conversion to the physiologically active molting hormone, 20-hydroxyecdysone Manduca sexta ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.22 E20-M
-
 Manduca sexta

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.99.22 NADPH preferred cofactor Manduca sexta

General Information

EC Number General Information Comment Organism
1.14.99.22 additional information physiological and developmental significance of the mitochondrial, NADPH-forming energy-linked transhydrogenations, that catalyzes a reversible hydride ion transfer between NADP(H) and NAD(H), for E20-M activity. NADPH-forming activity occurs as a nonenergy- or energy-linked activity with energy for the latter derived from either electron transport-dependent NADH or succinate utilization, or ATP hydrolysis by Mg2+-dependent ATPase, overview Manduca sexta
1.14.99.22 physiological function essential need for conversion of the molting hormone, ecdysone, to its physiologically active form, 20-hydroxyecdysone by E20-M during the post-embryonic development Manduca sexta