EC Number | Cloned (Comment) | Organism |
---|---|---|
1.11.1.21 | expressed in Escherichia coli UM2 cells | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.11.1.21 | S315T | the mutant is a competent catalase-peroxidase with reduced activity toward isoniazid. The catalase activity is reduced 6fold and the peroxidase activity is decreased less than 2fold compared with the activities for the wild type enzyme | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.21 | 0.07 | - |
H2O2 | mutant enzyme S315T, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
1.11.1.21 | 0.07 | - |
H2O2 | wild type enzyme, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
1.11.1.21 | 0.08 | - |
isoniazid | mutant enzyme S315T, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
1.11.1.21 | 0.43 | - |
isoniazid | wild type enzyme, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.21 | Mycobacterium tuberculosis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.21 | DEAE-Sepharose column chromatography, Sephacryl S300 gel filtration, and MonoQ HR5/5 column chromatography | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.21 | H2O2 | - |
Mycobacterium tuberculosis | O2 + H2O | - |
? | |
1.11.1.21 | isoniazid + H2O2 | - |
Mycobacterium tuberculosis | oxidized isoniazid + H2O | - |
? | |
1.11.1.21 | o-dianisidine + tert-butyl hydroperoxide | - |
Mycobacterium tuberculosis | o-dianisidine quinonediimine + ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.11.1.21 | KatG | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.21 | 0.057 | - |
isoniazid | wild type enzyme, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
1.11.1.21 | 0.081 | - |
isoniazid | mutant enzyme S315T, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
1.11.1.21 | 1.2 | - |
o-Dianisidine | mutant enzyme S315T, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
1.11.1.21 | 2 | - |
o-Dianisidine | wild type enzyme, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
1.11.1.21 | 2100 | - |
H2O2 | mutant enzyme S315T, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
1.11.1.21 | 5800 | - |
H2O2 | wild type enzyme, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.21 | heme | 1.1 hemes per subunit for wild type KatG | Mycobacterium tuberculosis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.21 | 19 | - |
o-Dianisidine | mutant enzyme S315T, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
1.11.1.21 | 32 | - |
o-Dianisidine | wild type enzyme, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
1.11.1.21 | 160 | - |
H2O2 | mutant enzyme S315T, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
1.11.1.21 | 1000 | - |
H2O2 | wild type enzyme, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis |