Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wengenack, N.L.; Uhl, J.R.; St Amand, A.L.; Tomlinson, A.J.; Benson, L.M.; Naylor, S.; Kline, B.C.; Cockerill, F.R.; Rusnak, F.
    Recombinant Mycobacterium tuberculosis KatG(S315T) is a competent catalase-peroxidase with reduced activity toward isoniazid (1997), J. Infect. Dis., 176, 722-727.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.21 expressed in Escherichia coli UM2 cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.21 S315T the mutant is a competent catalase-peroxidase with reduced activity toward isoniazid. The catalase activity is reduced 6fold and the peroxidase activity is decreased less than 2fold compared with the activities for the wild type enzyme Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.21 0.07
-
H2O2 mutant enzyme S315T, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
1.11.1.21 0.07
-
H2O2 wild type enzyme, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
1.11.1.21 0.08
-
isoniazid mutant enzyme S315T, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
1.11.1.21 0.43
-
isoniazid wild type enzyme, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.21 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.21 DEAE-Sepharose column chromatography, Sephacryl S300 gel filtration, and MonoQ HR5/5 column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.21 H2O2
-
Mycobacterium tuberculosis O2 + H2O
-
?
1.11.1.21 isoniazid + H2O2
-
Mycobacterium tuberculosis oxidized isoniazid + H2O
-
?
1.11.1.21 o-dianisidine + tert-butyl hydroperoxide
-
Mycobacterium tuberculosis o-dianisidine quinonediimine + ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.21 KatG
-
Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.21 0.057
-
isoniazid wild type enzyme, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
1.11.1.21 0.081
-
isoniazid mutant enzyme S315T, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
1.11.1.21 1.2
-
o-Dianisidine mutant enzyme S315T, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
1.11.1.21 2
-
o-Dianisidine wild type enzyme, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
1.11.1.21 2100
-
H2O2 mutant enzyme S315T, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
1.11.1.21 5800
-
H2O2 wild type enzyme, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.21 heme 1.1 hemes per subunit for wild type KatG Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.21 19
-
o-Dianisidine mutant enzyme S315T, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
1.11.1.21 32
-
o-Dianisidine wild type enzyme, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
1.11.1.21 160
-
H2O2 mutant enzyme S315T, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
1.11.1.21 1000
-
H2O2 wild type enzyme, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis