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Literature summary extracted from

  • Zhou, J.; Bao, L.; Chang, L.; Zhou, Y.; Lu, H.
    Biochemical and kinetic characterization of GH43 beta-D-xylosidase/alpha-L-arabinofuranosidase and GH30 alpha-L-arabinofuranosidase/beta-D-xylosidase from rumen metagenome (2012), J. Ind. Microbiol. Biotechnol., 39, 143-152.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.37 expressed in Escherichia coli BL21(DE3) cells uncultured bacterium
3.2.1.55 expressed in Escherichia coli BL21(DE3) cells uncultured rumen bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside
-
uncultured rumen bacterium
3.2.1.55 4-nitrophenyl beta-D-xylopyranoside
-
uncultured rumen bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 3.43
-
4-nitrophenyl beta-D-xylopyranoside in 50 mM phosphate/citrate buffer pH 7.0, at 40°C uncultured bacterium
3.2.1.55 2.23
-
4-nitrophenyl alpha-L-arabinofuranoside in 50 mM phosphate/citrate buffer pH 7.0, at 40°C uncultured rumen bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 42000
-
x * 42000, SDS-PAGE uncultured bacterium
3.2.1.55 42000
-
x * 42000, SDS-PAGE uncultured rumen bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 uncultured bacterium
-
from rumen of Bos grunniens
-
3.2.1.55 uncultured rumen bacterium E3TBJ3 from rumen of Bos grunniens
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 Ni-NTA resin column chromatography uncultured bacterium
3.2.1.55 Ni-NTA resin column chromatography uncultured rumen bacterium

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O highest activity uncultured bacterium 4-nitrophenol + beta-D-xylopyranose
-
?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
uncultured rumen bacterium 4-nitrophenol + alpha-L-arabinofuranose
-
?
3.2.1.55 additional information no activity on 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, rice straw xylan or birchwood xylan uncultured rumen bacterium ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 ? x * 42000, SDS-PAGE uncultured bacterium
3.2.1.55 ? x * 42000, SDS-PAGE uncultured rumen bacterium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 alpha-L-arabinofuranosidase
-
uncultured bacterium
3.2.1.37 beta-D-xylosidase/alpha-L-arabinofuranosidase
-
uncultured bacterium
3.2.1.37 RuXyn1
-
uncultured bacterium
3.2.1.55 alpha-L-arabinofuranosidase
-
uncultured rumen bacterium
3.2.1.55 beta-D-xylosidase/alpha-L-arabinofuranosidase
-
uncultured rumen bacterium
3.2.1.55 RuXyn1
-
uncultured rumen bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 40 50 the enzyme is relatively stable at 40°C, but loses 76% of its activity after 10 min at 45°C, and 97% of activity at 50°C. The irreversible thermal denaturation half-time of the enzyme is calculated to be 112 min at 40°C, and 5.8 min at 45°C uncultured rumen bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.37 76
-
4-nitrophenyl beta-D-xylopyranoside in 50 mM phosphate/citrate buffer pH 7.0, at 40°C uncultured bacterium
3.2.1.55 5.6
-
4-nitrophenyl alpha-L-arabinofuranoside in 50 mM phosphate/citrate buffer pH 7.0, at 40°C uncultured rumen bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 6.5 7.5
-
uncultured bacterium
3.2.1.55 6.5 7.5
-
uncultured rumen bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.55 4 8 the enzyme retains over 72% of optimal activity at pH 6.0, at least 40% at pH 5.0, and more than 10% at pH 4.0, however enzyme activity decreases dramatically at pH 8.0, and only 20% of its activity remains uncultured rumen bacterium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.55 20.8
-
4-nitrophenyl alpha-L-arabinofuranoside in 50 mM phosphate/citrate buffer pH 7.0, at 40°C uncultured rumen bacterium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.37 8.1
-
4-nitrophenyl beta-D-xylopyranoside in 50 mM phosphate/citrate buffer pH 7.0, at 40°C uncultured bacterium
3.2.1.55 11.7
-
4-nitrophenyl alpha-L-arabinofuranoside in 50 mM phosphate/citrate buffer pH 7.0, at 40°C uncultured rumen bacterium