EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.40 | L-rhamnose | compeitive | Acrostalagmus luteoalbus | |
3.2.1.40 | additional information | neither divalent cations such as Ca2+, Mg2+, Mn2+, and Co2+ nor reducing agents such as 2-mercaptoethanol and dithiothreitol show effects on enzyme activtiy | Acrostalagmus luteoalbus | |
3.2.1.40 | Zn2+ | 0.2 mM, complete inhibition | Acrostalagmus luteoalbus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.40 | 3.38 | - |
4-nitrophenyl-alpha-L-rhamnoside | pH 8.0, 55°C | Acrostalagmus luteoalbus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.40 | 109000 | - |
x * 109000, SDS-PAGE | Acrostalagmus luteoalbus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.40 | Acrostalagmus luteoalbus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.40 | from cells grown on rhamnose as sole carbon source | Acrostalagmus luteoalbus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.40 | 526 | - |
pH 9.5, 37°C | Acrostalagmus luteoalbus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.40 | 4-nitrophenyl alpha-L-rhamnoside + H2O | - |
Acrostalagmus luteoalbus | alpha-L-rhamnose + 4-nitrophenol | - |
? | |
3.2.1.40 | hesperidin + H2O | - |
Acrostalagmus luteoalbus | hesperetin 7-O-beta-D-glucoside + L-rhamnose | - |
? | |
3.2.1.40 | additional information | enzyme is able to hydrolyze alpha-1,2- and alpha-1,6-linkages to beta-D-glucosides in addition to direct linkages from the L-rhamnose residue to the aglycon | Acrostalagmus luteoalbus | ? | - |
? | |
3.2.1.40 | naringin + H2O | - |
Acrostalagmus luteoalbus | 4',5,7-trihydroxyflavanone 7-O-beta-D-glucoside + L-rhamnose | - |
? | |
3.2.1.40 | quercitrin + H2O | - |
Acrostalagmus luteoalbus | quercetin + L-rhamnose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.40 | ? | x * 109000, SDS-PAGE | Acrostalagmus luteoalbus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.40 | 55 | - |
- |
Acrostalagmus luteoalbus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.40 | 40 | - |
stable up to 4 h | Acrostalagmus luteoalbus |
3.2.1.40 | 55 | - |
4 h, 81% residual activity | Acrostalagmus luteoalbus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.40 | 8 | - |
- |
Acrostalagmus luteoalbus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.40 | 7.5 | 9 | 24 h, without substrate, 95% residual activity | Acrostalagmus luteoalbus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.40 | 4.7 | - |
L-rhamnose | pH 9.5, 37°C | Acrostalagmus luteoalbus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.40 | Acrostalagmus luteoalbus | isoelectric focusing | - |
4.6 |