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Literature summary extracted from

  • Rojas, N.L.; Voget, C.E.; Hours, R.A.; Cavalitto, S.F.
    Purification and characterization of a novel alkaline alpha-L-rhamnosidase produced by Acrostalagmus luteo albus (2011), J. Ind. Microbiol. Biotechnol., 38, 1515-1522.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.40 L-rhamnose compeitive Acrostalagmus luteoalbus
3.2.1.40 additional information neither divalent cations such as Ca2+, Mg2+, Mn2+, and Co2+ nor reducing agents such as 2-mercaptoethanol and dithiothreitol show effects on enzyme activtiy Acrostalagmus luteoalbus
3.2.1.40 Zn2+ 0.2 mM, complete inhibition Acrostalagmus luteoalbus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.40 3.38
-
 4-nitrophenyl-alpha-L-rhamnoside pH 8.0, 55°C Acrostalagmus luteoalbus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.40 109000
-
 x * 109000, SDS-PAGE Acrostalagmus luteoalbus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Acrostalagmus luteoalbus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40 from cells grown on rhamnose as sole carbon source Acrostalagmus luteoalbus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.40 526
-
 pH 9.5, 37°C Acrostalagmus luteoalbus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 4-nitrophenyl alpha-L-rhamnoside + H2O
-
 Acrostalagmus luteoalbus alpha-L-rhamnose + 4-nitrophenol
-
 ?
3.2.1.40 hesperidin + H2O
-
 Acrostalagmus luteoalbus hesperetin 7-O-beta-D-glucoside + L-rhamnose
-
 ?
3.2.1.40 additional information enzyme is able to hydrolyze alpha-1,2- and alpha-1,6-linkages to beta-D-glucosides in addition to direct linkages from the L-rhamnose residue to the aglycon Acrostalagmus luteoalbus ?
-
 ?
3.2.1.40 naringin + H2O
-
 Acrostalagmus luteoalbus 4',5,7-trihydroxyflavanone 7-O-beta-D-glucoside + L-rhamnose
-
 ?
3.2.1.40 quercitrin + H2O
-
 Acrostalagmus luteoalbus quercetin + L-rhamnose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.40 ? x * 109000, SDS-PAGE Acrostalagmus luteoalbus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.40 55
-
-
 Acrostalagmus luteoalbus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.40 40
-
 stable up to 4 h Acrostalagmus luteoalbus
3.2.1.40 55
-
 4 h, 81% residual activity Acrostalagmus luteoalbus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 8
-
-
 Acrostalagmus luteoalbus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.40 7.5 9 24 h, without substrate, 95% residual activity Acrostalagmus luteoalbus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.40 4.7
-
 L-rhamnose pH 9.5, 37°C Acrostalagmus luteoalbus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.40 Acrostalagmus luteoalbus isoelectric focusing
-
 4.6