Literature summary extracted from

Sosa, A.C.; Espejo, A.J.; Rodriguez, E.A.; Lizaraso, L.M.; Rojas, A.; Guevara, J.; Echeverri, O.Y.; Barrera, L.A.
Development of a sandwich enzyme linked immunosorbent assay (ELISA) for the quantification of iduronate-2-sulfate sulfatase (2011), J. Immunol. Methods, 368, 64-70.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.6.13	development of an expression system for human recombinant IDS in Pichia pastoris and of a detection method for enzyme detection during production and purification processes, which can be used also to measure the enzyme in human fluids, immunoquantification assay using rabbit IgG and chicken IgY, overview	Homo sapiens

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.6.13	Homo sapiens	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.1.6.13	commercial preparation	recombinant enzyme	Homo sapiens	-
3.1.6.13	additional information	development of an ELISA system to detect and quantify IDS with IgY antibodies, overview	Homo sapiens	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.1.6.13	0.000006	0.00066	hrIDS detection by ELISA-sandwich and fluorometric assay in Pichia pastoris culture samples, substrate is 4-methylumbelliferyl-2-iduronate-2-sulfate, pH not specified in the publication, temperature not specified in the publication, overview	Homo sapiens
3.1.6.13	0.00063	0.0021	enzyme determination in human plasma samples by a Fluorometric method, and ELISA double sandwich technique after several freeze and thawing cycles, substrate is 4-methylumbelliferyl-2-iduronate-2-sulfate, pH not specified in the publication, temperature not specified in the publication, overview	Homo sapiens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.6.13	4-methylumbelliferyl-alpha-L-iduronate 2-sulfate + H2O	artificial substrate	Homo sapiens	4-methylumbelliferol + L-iduronate 2-sulfate	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.6.13	iduronate-2-sulfate sulfatase	-	Homo sapiens

General Information

EC Number	General Information	Comment	Organism
3.1.6.13	malfunction	iduronate-2-sulfate sulfatase, IDS, deficiency causes the Hunter syndrome ormucopolysaccharidosis type II	Homo sapiens
3.1.6.13	additional information	development of an expression system for human recombinant IDS in Pichia pastoris and of a detection method for enzyme detection during production and purification processes, which can be used also to measure the enzyme in human fluids, immunoquantification assay using rabbit IgG and chicken IgY, overview	Homo sapiens

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Release 2023.1 (January 2023)