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Literature summary extracted from
- Pp6-FEH1 encodes an enzyme for degradation of highly polymerized levan and is transcriptionally induced by defoliation in timothy (Phleum pratense L.) (2011), J. Exp. Bot., 62, 3421-3431.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.80 | expressed in Pichia pastoris | Phleum pratense |
| 3.2.1.154 | expressed in Pichia pastoris strain X-33 | Phleum pratense |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.80 | sucrose | the inhibitory effect of sucrose is 4% at 10 mM sucrose and 23% at 100 mM sucrose | Phleum pratense |  |
| 3.2.1.154 | sucrose | the inhibitory effect of sucrose is 4% at 10 mM sucrose and 23% at 100 mM sucrose | Phleum pratense | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.154 | vacuole | - |
Phleum pratense | 5773 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.80 | 63400 | - |
x * 63400, calculated from amino acid sequence | Phleum pratense |
| 3.2.1.80 | 78000 | - |
x * 78000, SDS-PAGE | Phleum pratense |
| 3.2.1.154 | 63400 | - |
x * 63400, putative mature protein, calculated from amino acid sequence | Phleum pratense |
| 3.2.1.154 | 78000 | - |
x * 78000, recombinant enzyme, SDS-PAGE | Phleum pratense |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.80 | Phleum pratense | - |
cultivar Hokushu | - |
| 3.2.1.154 | Phleum pratense | E9RGV6 | cultivar Hokushu | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.154 | glycoprotein | the enzyme has five N-glycosylation sites | Phleum pratense |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.80 | plant crown | - |
Phleum pratense | - |
| 3.2.1.154 | seedling | - |
Phleum pratense | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.80 | 1,1,1-kestopentaose + H2O | 18% activity compared to levan | Phleum pratense | ? | - |
? | |
| 3.2.1.80 | 1,1-kestotetraose + H2O | 18% activity compared to levan | Phleum pratense | ? | - |
? | |
| 3.2.1.80 | 1-kestotriose + H2O | 7% activity compared to levan | Phleum pratense | ? | - |
? | |
| 3.2.1.80 | 6-kestotriose + H2O | 25% activity compared to levan | Phleum pratense | ? | - |
? | |
| 3.2.1.80 | inulin + 5 H2O | 6% activity compared to levan | Phleum pratense | 5 beta-D-fructofuranose + alpha-D-glucopyranose | - |
? | |
| 3.2.1.80 | levan + H2O | 100% activity | Phleum pratense | beta-D-fructose + ? | - |
? | |
| 3.2.1.80 | additional information | the recombinant enzyme completely degrades fructans that are composed mainly of beta(2,6)-linked and linear fructans (levan) with a high degree of polymerization. The enzyme has significant activity against beta(2,1)-linked fructans, but considerably less than against beta(2,6)-linked fructans. The enzyme also has weak invertase activity | Phleum pratense | ? | - |
? | |
| 3.2.1.80 | sucrose + H2O | less than 1%, 2% and 15% activity towards 1 mM, 10 mM and 100 mM sucrose compared to levan | Phleum pratense | D-fructose + D-glucose | - |
? | |
| 3.2.1.154 | 1,1,1-kestopentaose + H2O | 18% activity compared to levan | Phleum pratense | ? | - |
? | |
| 3.2.1.154 | 1,1-kestotetraose + H2O | 18% activity compared to levan | Phleum pratense | ? | - |
? | |
| 3.2.1.154 | 1-kestotriose + H2O | 7% activity compared to levan | Phleum pratense | ? | - |
? | |
| 3.2.1.154 | 6-kestotriose + H2O | 25% activity compared to levan | Phleum pratense | ? | - |
? | |
| 3.2.1.154 | inulin + H2O | 6% activity compared to levan | Phleum pratense | ? | - |
? | |
| 3.2.1.154 | levan + H2O | 100% activity, the enzyme shows 6-FEH activity against levan (mean degree of polymerization is 20) that is 4fold higher than against 6-kestotriose(mean degree of polymerization is 3) | Phleum pratense | ? | - |
? | |
| 3.2.1.154 | additional information | the enzyme has significant activity against beta(2,1)-linked fructans, but considerably less than against beta(2,6)-linked fructans and shows weak invertase activity | Phleum pratense | ? | - |
? | |
| 3.2.1.154 | sucrose + H2O | 15% activity with 100 mM sucrose and less than 1% activity with 1 mM sucrose compared to levan | Phleum pratense | D-fructose + D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.80 | ? | x * 78000, SDS-PAGE | Phleum pratense |
| 3.2.1.80 | ? | x * 63400, calculated from amino acid sequence | Phleum pratense |
| 3.2.1.154 | ? | x * 63400, putative mature protein, calculated from amino acid sequence | Phleum pratense |
| 3.2.1.154 | ? | x * 78000, recombinant enzyme, SDS-PAGE | Phleum pratense |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.80 | FEH1 | - |
Phleum pratense |
| 3.2.1.80 | fructan 1-exohydrolase | - |
Phleum pratense |
| 3.2.1.154 | 6-FEH1 | - |
Phleum pratense |
| 3.2.1.154 | fructan 6-exohydrolase | - |
Phleum pratense |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.80 | 30 | - |
- |
Phleum pratense |
| 3.2.1.154 | 30 | - |
about 30°C | Phleum pratense |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.154 | 4 | 30 | the activity of the enzyme remains relatively high even at 4°C, with up to 38% of its activity at 30°C | Phleum pratense |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.80 | 4 | - |
the activity of the enzyme remains relatively high even at 4°C, with up to 38% of its activity at 30°C | Phleum pratense |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.80 | 5.5 | - |
- |
Phleum pratense |
| 3.2.1.154 | 5.5 | - |
- |
Phleum pratense |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.80 | Phleum pratense | calculated from amino acid sequence | - |
5 |
| 3.2.1.154 | Phleum pratense | putative mature protein, calculated from amino acid sequence | - |
5 |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.2.1.80 | Phleum pratense | in the stubble of seedlings and in young haplocorms from adult timothy plants, enzyme transcripts are significantly increased within 3 h of defoliation | up |
| 3.2.1.154 | Phleum pratense | in the stubble of seedlings and in young haplocorms from adult timothy plants, transcripts of 6-FEH1 are significantly increased (about 7fold) within 3 h of defoliation, followed by an increase in fructan 6-exohydrolase activity | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.1.154 | physiological function | the enzyme plays a role in the degradation of fructans and the mobilization of carbon sources for regrowth after defoliation in timothy | Phleum pratense |
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