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Characterization and inhibition studies of an alpha-carbonic anhydrase from the endangered sturgeon species Acipenser gueldenstaedti

Kolayli, S.; Karahalil, F.; Sahin, H.; Dincer, B.; Supuran, C.T.; J. Enzyme Inhib. Med. Chem. 26, 895-900 (2011)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.1
2,4-Dichlorophenol
-
Acipenser gueldenstaedtii
4.2.1.1
acetazolamide
strong inhibition
Acipenser gueldenstaedtii
4.2.1.1
Ag+
weak inhibition
Acipenser gueldenstaedtii
4.2.1.1
Carbaryl
-
Acipenser gueldenstaedtii
4.2.1.1
Co2+
weak inhibition
Acipenser gueldenstaedtii
4.2.1.1
Cu2+
weak inhibition
Acipenser gueldenstaedtii
4.2.1.1
maneb
-
Acipenser gueldenstaedtii
4.2.1.1
parathion
-
Acipenser gueldenstaedtii
4.2.1.1
propineb
-
Acipenser gueldenstaedtii
4.2.1.1
sulphanilamide
strong inhibition
Acipenser gueldenstaedtii
4.2.1.1
Zn2+
weak inhibition
Acipenser gueldenstaedtii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.1
additional information
-
additional information
Km is 4.0 mM with 4-nitrophenyl acetate, pH 9.0, 30C, esterase activity
Acipenser gueldenstaedtii
4.2.1.1
10.9
-
CO2
pH 7.4, 30C, CO2 hydration activity
Acipenser gueldenstaedtii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.1
29000
-
x * 29000, SDS-PAGE
Acipenser gueldenstaedtii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.1
H2CO3
Acipenser gueldenstaedtii
-
CO2 + H2O
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.1
Acipenser gueldenstaedtii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.1
native enzyme 539fold by one-step affinity chromatography on L-tyrosine sulphonamide
Acipenser gueldenstaedtii
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.1
erythrocyte
-
Acipenser gueldenstaedtii
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.2.1.1
26970
-
purified native enzyme, pH 7.4, 30C
Acipenser gueldenstaedtii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.1
H2CO3
-
715746
Acipenser gueldenstaedtii
CO2 + H2O
-
-
-
r
4.2.1.1
additional information
the enzyme shows esterase activity with 4-nitrophenyl acetate
715746
Acipenser gueldenstaedtii
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.1
?
x * 29000, SDS-PAGE
Acipenser gueldenstaedtii
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.1
30
-
assay at
Acipenser gueldenstaedtii
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.1
additional information
-
additional information
20.3/s with 4-nitrophenyl acetate, pH 9.0, 30C, esterase activity
Acipenser gueldenstaedtii
4.2.1.1
1200000
-
CO2
pH 7.4, 30C, CO2 hydration activity
Acipenser gueldenstaedtii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.1
7.4
-
assay at
Acipenser gueldenstaedtii
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.1
0.000026
-
acetazolamide
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
4.2.1.1
0.00005
-
acetazolamide
pH 9.0, 30C, inhibition of esterase activity with 4-nitrophenyl acetate
Acipenser gueldenstaedtii
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
4.2.1.1
0.0001
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
acetazolamide
4.2.1.1
0.004
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
sulphanilamide
4.2.1.1
0.016
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
propineb
4.2.1.1
0.018
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
maneb
4.2.1.1
0.102
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
parathion
4.2.1.1
0.24
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
2,4-Dichlorophenol
4.2.1.1
0.398
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
Carbaryl
4.2.1.1
1.7
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
Ag+
4.2.1.1
2.8
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
Zn2+
4.2.1.1
3
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
Co2+
4.2.1.1
5.2
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
Cu2+
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
4.2.1.1
0.0001
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
acetazolamide
4.2.1.1
0.004
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
sulphanilamide
4.2.1.1
0.016
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
propineb
4.2.1.1
0.018
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
maneb
4.2.1.1
0.102
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
parathion
4.2.1.1
0.24
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
2,4-Dichlorophenol
4.2.1.1
0.398
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
Carbaryl
4.2.1.1
1.7
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
Ag+
4.2.1.1
2.8
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
Zn2+
4.2.1.1
3
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
Co2+
4.2.1.1
5.2
-
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
Cu2+
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.1
2,4-Dichlorophenol
-
Acipenser gueldenstaedtii
4.2.1.1
acetazolamide
strong inhibition
Acipenser gueldenstaedtii
4.2.1.1
Ag+
weak inhibition
Acipenser gueldenstaedtii
4.2.1.1
Carbaryl
-
Acipenser gueldenstaedtii
4.2.1.1
Co2+
weak inhibition
Acipenser gueldenstaedtii
4.2.1.1
Cu2+
weak inhibition
Acipenser gueldenstaedtii
4.2.1.1
maneb
-
Acipenser gueldenstaedtii
4.2.1.1
parathion
-
Acipenser gueldenstaedtii
4.2.1.1
propineb
-
Acipenser gueldenstaedtii
4.2.1.1
sulphanilamide
strong inhibition
Acipenser gueldenstaedtii
4.2.1.1
Zn2+
weak inhibition
Acipenser gueldenstaedtii
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.1
0.000026
-
acetazolamide
pH 7.4, 30C, inhibition of CO2 hydration activity
Acipenser gueldenstaedtii
4.2.1.1
0.00005
-
acetazolamide
pH 9.0, 30C, inhibition of esterase activity with 4-nitrophenyl acetate
Acipenser gueldenstaedtii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.1
additional information
-
additional information
Km is 4.0 mM with 4-nitrophenyl acetate, pH 9.0, 30C, esterase activity
Acipenser gueldenstaedtii
4.2.1.1
10.9
-
CO2
pH 7.4, 30C, CO2 hydration activity
Acipenser gueldenstaedtii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.1
29000
-
x * 29000, SDS-PAGE
Acipenser gueldenstaedtii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.1
H2CO3
Acipenser gueldenstaedtii
-
CO2 + H2O
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.1
native enzyme 539fold by one-step affinity chromatography on L-tyrosine sulphonamide
Acipenser gueldenstaedtii
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.1
erythrocyte
-
Acipenser gueldenstaedtii
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.2.1.1
26970
-
purified native enzyme, pH 7.4, 30C
Acipenser gueldenstaedtii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.1
H2CO3
-
715746
Acipenser gueldenstaedtii
CO2 + H2O
-
-
-
r
4.2.1.1
additional information
the enzyme shows esterase activity with 4-nitrophenyl acetate
715746
Acipenser gueldenstaedtii
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.1
?
x * 29000, SDS-PAGE
Acipenser gueldenstaedtii
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.1
30
-
assay at
Acipenser gueldenstaedtii
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.1
additional information
-
additional information
20.3/s with 4-nitrophenyl acetate, pH 9.0, 30C, esterase activity
Acipenser gueldenstaedtii
4.2.1.1
1200000
-
CO2
pH 7.4, 30C, CO2 hydration activity
Acipenser gueldenstaedtii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.1
7.4
-
assay at
Acipenser gueldenstaedtii
General Information
EC Number
General Information
Commentary
Organism
4.2.1.1
additional information
the wide use of dithiocarbamate pesticides may be one of the factors enhancing the vulnerability of this sturgeon species to pollutants, since they act as effective carbonate dehydratase inhibitors
Acipenser gueldenstaedtii
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.2.1.1
additional information
the wide use of dithiocarbamate pesticides may be one of the factors enhancing the vulnerability of this sturgeon species to pollutants, since they act as effective carbonate dehydratase inhibitors
Acipenser gueldenstaedtii