Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bayrak, A.; Bayrak, T.; Demirpence, E.; Kilinc, K.
    Differential hydrolysis of homocysteine thiolactone by purified human serum (192)Q and (192)R PON1 isoenzymes (2011), J. Chromatogr. B, 879, 49-55.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.2 0.92
-
 phenyl acetate isozyme 192R, in 50 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 25°C Homo sapiens
3.1.1.2 0.98
-
 phenyl acetate isozyme 192Q, in 50 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 25°C Homo sapiens
3.1.1.25 21.2
-
 homocysteine thiolactone isozyme 192R, in 50 mM HEPES (pH 7.4), 37°C Homo sapiens
3.1.1.25 23.5
-
 homocysteine thiolactone isozyme 192Q, in 50 mM HEPES (pH 7.4), 37°C Homo sapiens
3.1.8.1 0.56
-
 paraoxon isozyme 192R, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C Homo sapiens
3.1.8.1 0.96
-
 paraoxon isozyme 192Q, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.2 45000
-
 x * 45000, SDS-PAGE Homo sapiens
3.1.1.25 45000
-
 x * 45000, SDS-PAGE Homo sapiens
3.1.8.1 45000
-
 x * 45000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.25 homocysteine thiolactone + H2O Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.2 Homo sapiens
-
-
-
3.1.1.25 Homo sapiens
-
-
-
3.1.8.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.2 ammonium sulfate precipitation, DEAE-Trisacryl M column chromatography, Sephacryl S300HR gel filtration, and Cibacron Blue 3GA gel filtration Homo sapiens
3.1.1.25 ammonium sulfate precipitation, DEAE-Trisacryl M column chromatography, Sephacryl S300HR gel filtration, and Cibacron Blue 3GA gel filtration Homo sapiens
3.1.8.1 ammonium sulfate precipitation, DEAE-Trisacryl M column chromatography, Sephacryl S300HR gel filtration, and Cibacron Blue 3GA gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.2 serum
-
 Homo sapiens
-
3.1.1.25 serum
-
 Homo sapiens
-
3.1.8.1 serum
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.2 1.22
-
 isozyme 192Q, crude enzyme, using phenyl acetate as substrate, in 50 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 25°C Homo sapiens
3.1.1.2 1.34
-
 isozyme 192R, crude enzyme, using phenyl acetate as substrate, in 50 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 25°C Homo sapiens
3.1.1.2 970
-
 isozyme 192Q, after 795fold purification, using phenyl acetate as substrate, in 50 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 25°C Homo sapiens
3.1.1.2 1048
-
 isozyme 192R, after 783fold purification, using phenyl acetate as substrate, in 50 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 25°C Homo sapiens
3.1.8.1 1.88
-
 isozyme 192Q, crude enzyme, using paraoxon as substrate, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C Homo sapiens
3.1.8.1 4.34
-
 isozyme 192Q, after 589.7fold purification, using paraoxon as substrate, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C Homo sapiens
3.1.8.1 1168
-
 isozyme 192R, crude enzyme, using paraoxon as substrate, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C Homo sapiens
3.1.8.1 3221
-
 isozyme 192R, after 742fold purification, using paraoxon as substrate, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.2 phenyl acetate + H2O
-
 Homo sapiens phenol + acetate
-
 ?
3.1.1.25 homocysteine thiolactone + H2O
-
 Homo sapiens ?
-
 ?
3.1.8.1 paraoxon + H2O i.e. O,O'-diethyl-4-nitrophenyl phosphothioate Homo sapiens 4-nitrophenol + diethyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.2 ? x * 45000, SDS-PAGE Homo sapiens
3.1.1.25 ? x * 45000, SDS-PAGE Homo sapiens
3.1.8.1 ? x * 45000, SDS-PAGE Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.2 0.000133
-
 phenyl acetate isozyme 192R, in 50 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 25°C Homo sapiens
3.1.1.2 0.98
-
 phenyl acetate isozyme 192Q, in 50 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 25°C Homo sapiens
3.1.1.25 1.11
-
 homocysteine thiolactone isozyme 192Q, in 50 mM HEPES (pH 7.4), 37°C Homo sapiens
3.1.1.25 21.2
-
 homocysteine thiolactone isozyme 192R, in 50 mM HEPES (pH 7.4), 37°C Homo sapiens
3.1.8.1 1.11
-
 paraoxon isozyme 192Q, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C Homo sapiens
3.1.8.1 3.67
-
 paraoxon isozyme 192R, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C Homo sapiens