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Literature summary extracted from

  • Kara, H.E.; Sinan, S.; Turan, Y.
    Purification of beta-glucosidase from olive (Olea europaea L.) fruit tissue with specifically designed hydrophobic interaction chromatography and characterization of the purified enzyme (2011), J. Chromatogr. B, 879, 1507-1512.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Ag+ inhibits activity Olea europaea
3.2.1.21 Cd2+ inhibits activity Olea europaea
3.2.1.21 Cu2+ inhibits activity Olea europaea
3.2.1.21 D-glucono-1,5-lactone
-
 Olea europaea
3.2.1.21 D-glucose
-
 Olea europaea
3.2.1.21 Ni2+ inhibits activity Olea europaea
3.2.1.21 Pb2+ inhibits activity Olea europaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 2.22
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.5, 37°C Olea europaea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 Fe2+ increases activity Olea europaea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 65000
-
 SDS-PAGE Olea europaea

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Olea europaea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 purified by salting out with ammonium sulfate and using specifically designed Sepharose-4B-l-tyrosine-1-naphthylamine hydrophobic interaction chromatography. Purification: 155fold, enzyme yield: 54% Olea europaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Olea europaea 2-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Olea europaea 4-nitrophenol + D-glucopyranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 beta-glucosidase
-
 Olea europaea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.21 0.000104
-
 Ni2+ pH 5.5, 37°C Olea europaea
3.2.1.21 0.000226
-
 Cu2+ pH 5.5, 37°C Olea europaea
3.2.1.21 0.000429
-
 Cd2+ pH 5.5, 37°C Olea europaea
3.2.1.21 0.00321
-
 Ag+ pH 5.5, 37°C Olea europaea
3.2.1.21 0.0126
-
 Pb2+ pH 5.5, 37°C Olea europaea
3.2.1.21 0.016
-
 D-glucono-1,5-lactone pH 5.5, 37°C Olea europaea
3.2.1.21 6.4
-
 D-glucose pH 5.5, 37°C Olea europaea

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.21 0.000038
-
 pH 5.5, 37°C Olea europaea Cd2+
3.2.1.21 0.000063
-
 pH 5.5, 37°C Olea europaea Ni2+
3.2.1.21 0.00061
-
 pH 5.5, 37°C Olea europaea Cu2+
3.2.1.21 0.00334
-
 pH 5.5, 37°C Olea europaea Ag+
3.2.1.21 0.0053
-
 pH 5.5, 37°C Olea europaea Pb2+
3.2.1.21 0.23
-
 pH 5.5, 37°C Olea europaea D-glucono-1,5-lactone
3.2.1.21 105.5
-
 pH 5.5, 37°C Olea europaea D-glucose