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Literature summary extracted from

  • Liang, T.; Kuo, Y.; Wu, P.; Wang, C.; Dzung, N.; Wang, S.
    Purification and characterization of a chitosanase and a protease by conversion of shrimp shell wastes fermented by Serratia marcescens subsp. sakuensis TKU019 (2010), J. Chin. Chem. Soc., 57, 857-863.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 Ca2+ 95% residual activity at 5 mM Serratia marcescens
3.2.1.132 Cu2+ 68% residual activity at 5 mM Serratia marcescens
3.2.1.132 Fe2+ 92% residual activity at 5 mM Serratia marcescens
3.2.1.132 Mn2+ 2% residual activity at 5 mM Serratia marcescens
3.2.1.132 additional information not influenced by 5 mM phenylmethylsulfonyl fluoride, 0.5 mM SDS, 0.5-2% (v/v) Tween 20, 0.5-2% (v/v) Tween 40, and 0.5-2% (v/v) Triton X-100 Serratia marcescens
3.2.1.132 SDS 57% residual activity at 2 mM Serratia marcescens
3.2.1.132 Zn2+ 72% residual activity at 5 mM Serratia marcescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.132 additional information not influenced by 5 mM Ca2+ Serratia marcescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 35000
-
 gel filtration Serratia marcescens
3.2.1.132 36000
-
 1 * 36000, SDS-PAGE Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Serratia marcescens
-
-
-
3.2.1.132 Serratia marcescens TKU019
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132 ammonium sulfate precipitation, DEAE-Sepharose CL-6B column chromatography and Sephacryl S-100 gel filtration Serratia marcescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 0.006
-
 culture supernatant, at pH 7.0 and 60°C Serratia marcescens
3.2.1.132 0.18
-
 after 30fold purification, at pH 7.0 and 60°C Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 beta-chitin + H2O 85% activity with chitin (beta-type) compared to chitosan (95% DD) Serratia marcescens ?
-
 ?
3.2.1.132 beta-chitin + H2O 85% activity with chitin (beta-type) compared to chitosan (95% DD) Serratia marcescens TKU019 ?
-
 ?
3.2.1.132 chitosan + H2O 100% activity with chitosan (95% degree of deacetylation), 26% activity with chitosan (85% degree of deacetylation) Serratia marcescens ?
-
 ?
3.2.1.132 chitosan + H2O 100% activity with chitosan (95% degree of deacetylation), 26% activity with chitosan (85% degree of deacetylation) Serratia marcescens TKU019 ?
-
 ?
3.2.1.132 additional information the enzyme shows no activity with chitosan (73% and 60% degrees of deacetylation), glycols chitosan, and alpha-chitin Serratia marcescens ?
-
 ?
3.2.1.132 additional information the enzyme shows no activity with chitosan (73% and 60% degrees of deacetylation), glycols chitosan, and alpha-chitin Serratia marcescens TKU019 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 monomer 1 * 36000, SDS-PAGE Serratia marcescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 60
-
-
 Serratia marcescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 60 80 the chitosanase maintains its initial activity at less than 60°C, the chitosanase is inactivated completely at 80°C Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 7
-
-
 Serratia marcescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.132 4 7
-
 Serratia marcescens

Expression

EC Number Organism Comment Expression
3.2.1.132 Serratia marcescens the chitosanase is suppressed by the simultaneously existing protease which also shows the maximum activity at the third day of incubation with shrimp shell as the sole carbon/nitrogen source down