BRENDA - Enzyme Database

Biochemical characterisation of aconitase from Corynebacterium glutamicum

Baumgart, M.; Bott, M.; J. Biotechnol. 154, 163-170 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.3
expression of N-terminally His-tagged aconitase in Escherichia coli strain BL21(DE3), alternative expression method uses overexpression of aconitase from plasmid pTYB2-acn, resulting in an aconitase containing only an additional glycine residue at the C-terminus
Corynebacterium glutamicum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.3
additional information
-
additional information
Michaelis-Menten kinetics
Corynebacterium glutamicum
4.2.1.3
0.0185
-
cis-aconitate
pH 8.0, 30°C
Corynebacterium glutamicum
4.2.1.3
0.48
-
citrate
pH 8.0, 30°C
Corynebacterium glutamicum
4.2.1.3
0.552
-
isocitrate
pH 8.0, 30°C
Corynebacterium glutamicum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.3
102300
-
1 * 104800, recombinant N-terminally His-tagged aconitase, sequence calculation, 1 * 102300, recombinant aconitase with an additional Gly at the C-terminus, sequence calculation
Corynebacterium glutamicum
4.2.1.3
114000
-
recombinant N-terminally His-tagged aconitase, gel filtration
Corynebacterium glutamicum
4.2.1.3
122000
-
recombinant aconitase with an additional Gly at the C-terminus, gel filtration
Corynebacterium glutamicum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.3
citrate
Corynebacterium glutamicum
-
cis-aconitate + H2O
-
-
r
4.2.1.3
citrate
Corynebacterium glutamicum ATCC 13032
-
cis-aconitate + H2O
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.3
Corynebacterium glutamicum
-
-
-
4.2.1.3
Corynebacterium glutamicum ATCC 13032
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.3
recombinant His-tagged aconitase from Escherichia coli strain BL21(DE3) by nickel affinty chromatography and gel filtration, removal of the His tag by factor Xa and further purification, or purification of recombinant aconitase, expressed from plasmid pTYB2-acn in Escherichia coli, using chitin beads, overview
Corynebacterium glutamicum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.3
culture condition:glucose-grown cell
-
Corynebacterium glutamicum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.3
0.206
-
dehydration of citrate to cis-aconitate, pH 8.0, 30°C
Corynebacterium glutamicum
4.2.1.3
0.266
-
dehydration of isocitrate to cis-aconitate, pH 8.0, 30°C
Corynebacterium glutamicum
4.2.1.3
0.433
-
reverse reaction, hydration of citrate or isocitrate, pH 8.0, 30°C
Corynebacterium glutamicum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
citrate
-
715699
Corynebacterium glutamicum
cis-aconitate + H2O
-
-
-
r
4.2.1.3
citrate
-
715699
Corynebacterium glutamicum ATCC 13032
cis-aconitate + H2O
-
-
-
r
4.2.1.3
isocitrate
-
715699
Corynebacterium glutamicum
cis-aconitate + H2O
-
-
-
r
4.2.1.3
isocitrate
-
715699
Corynebacterium glutamicum ATCC 13032
cis-aconitate + H2O
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.3
monomer
1 * 104800, recombinant N-terminally His-tagged aconitase, sequence calculation, 1 * 102300, recombinant aconitase with an additional Gly at the C-terminus, sequence calculation
Corynebacterium glutamicum
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.3
50
-
-
Corynebacterium glutamicum
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.2.1.3
20
65
-
Corynebacterium glutamicum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
7.5
7.75
-
Corynebacterium glutamicum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.3
5
10
-
Corynebacterium glutamicum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.3
expression of N-terminally His-tagged aconitase in Escherichia coli strain BL21(DE3), alternative expression method uses overexpression of aconitase from plasmid pTYB2-acn, resulting in an aconitase containing only an additional glycine residue at the C-terminus
Corynebacterium glutamicum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.3
additional information
-
additional information
Michaelis-Menten kinetics
Corynebacterium glutamicum
4.2.1.3
0.0185
-
cis-aconitate
pH 8.0, 30°C
Corynebacterium glutamicum
4.2.1.3
0.48
-
citrate
pH 8.0, 30°C
Corynebacterium glutamicum
4.2.1.3
0.552
-
isocitrate
pH 8.0, 30°C
Corynebacterium glutamicum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.3
102300
-
1 * 104800, recombinant N-terminally His-tagged aconitase, sequence calculation, 1 * 102300, recombinant aconitase with an additional Gly at the C-terminus, sequence calculation
Corynebacterium glutamicum
4.2.1.3
114000
-
recombinant N-terminally His-tagged aconitase, gel filtration
Corynebacterium glutamicum
4.2.1.3
122000
-
recombinant aconitase with an additional Gly at the C-terminus, gel filtration
Corynebacterium glutamicum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.3
citrate
Corynebacterium glutamicum
-
cis-aconitate + H2O
-
-
r
4.2.1.3
citrate
Corynebacterium glutamicum ATCC 13032
-
cis-aconitate + H2O
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.3
recombinant His-tagged aconitase from Escherichia coli strain BL21(DE3) by nickel affinty chromatography and gel filtration, removal of the His tag by factor Xa and further purification, or purification of recombinant aconitase, expressed from plasmid pTYB2-acn in Escherichia coli, using chitin beads, overview
Corynebacterium glutamicum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.3
culture condition:glucose-grown cell
-
Corynebacterium glutamicum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.3
0.206
-
dehydration of citrate to cis-aconitate, pH 8.0, 30°C
Corynebacterium glutamicum
4.2.1.3
0.266
-
dehydration of isocitrate to cis-aconitate, pH 8.0, 30°C
Corynebacterium glutamicum
4.2.1.3
0.433
-
reverse reaction, hydration of citrate or isocitrate, pH 8.0, 30°C
Corynebacterium glutamicum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
citrate
-
715699
Corynebacterium glutamicum
cis-aconitate + H2O
-
-
-
r
4.2.1.3
citrate
-
715699
Corynebacterium glutamicum ATCC 13032
cis-aconitate + H2O
-
-
-
r
4.2.1.3
isocitrate
-
715699
Corynebacterium glutamicum
cis-aconitate + H2O
-
-
-
r
4.2.1.3
isocitrate
-
715699
Corynebacterium glutamicum ATCC 13032
cis-aconitate + H2O
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.3
monomer
1 * 104800, recombinant N-terminally His-tagged aconitase, sequence calculation, 1 * 102300, recombinant aconitase with an additional Gly at the C-terminus, sequence calculation
Corynebacterium glutamicum
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.3
50
-
-
Corynebacterium glutamicum
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.2.1.3
20
65
-
Corynebacterium glutamicum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
7.5
7.75
-
Corynebacterium glutamicum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.3
5
10
-
Corynebacterium glutamicum